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Product Details
Source
Alexa Fluor 488-Labeled Monoclonal Anti-Whitlow 218 linker Antibody, Rabbit IgG (P3C09) is a monoclonal antibody recombinantly expressed from HEK293 cells, which provides higher batch consistency and long term security of supply.
Application
Flow Cytometry (Evaluation of CAR and SCFV containing 218 linker on Human cells).
Clone
P3C09
Species
Rabbit
Isotype
Rabbit IgG | Rabbit Kappa
Specificity
This product is a specific antibody specifically reacts with CAR or scFv containing the 218 linker sequence.
Reactivity
Human
Immunogen
Purified 218 linker protein
Conjugate
AF488
Excitation Wavelength: 488 nm
Emission Wavelength: 517 nm
Recommended Dilution
1:25
Formulation
Lyophilized from a 0.22 μm-filtered solution in PBS (pH 7.4) containing 0.03% ProClin 300, with trehalose as protectant.
Please contact us for customized product forms or formulations.
Reconstitution
Please refer to the Certificate of Analysis (CoA) for specific instructions.
For best performance, we strongly recommend following the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored in a lyophilized state at -20°C or lower.
Please protect from light and avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 24 months in lyophilized state;
- -70°C for 12 months after reconstitution.
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Performance Data
Bioactivity-FACS
Flow cytometric analysis of Anti-BCMA (C11D5.3) CAR 293 cells staining with Alexa Fluor 488-Labeled Monoclonal Anti-Whitlow 218 linker Antibody, Rabbit IgG (P3C09) (0.03% Proclin) (Cat. No. 218-AFFMY3) at 1:25 dilution (4 μL of the antibody stock solution corresponds to labeling of 1e6 cells in a final volume of 100 µL). Alexa Fluor 488 signal was used to evaluate the binding activity (QC tested).
Protocol
Non-specificity of Alexa Fluor 488-Labeled Monoclonal Anti-Whitlow 218 linker Antibody, Rabbit IgG (P3C09) (0.03% Proclin) (Cat. No. 218-AFFMY3) binding to CD3+ cells present in human PBMC. 5e5 of human PBMCs were simultaneously stained with PE-labeled anti-CD3 antibody and Alexa Fluor 488-Labeled Monoclonal Anti-Whitlow 218 linker Antibody (4 μL of the antibody stock solution corresponds to labeling of 5e5 cells in a final volume of 100 µL) and washed and then analyzed with FACS. Both Alexa Fluor 488 and PE positive signals was used to evaluate the non-specific binding activity to human CD3+ cells (QC tested).
Protocol
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