Product Details
Features and Advantages
- Native Conformation: Native sequences, tag-free and natural function.
- Reliable Activity: Biological activity calibrated against WHO/NIBSC standards.
- Stringent Quality Control: Protein content, purity, and cell-based bioactivity testing for each batch.
- Lowest Endotoxin level (<0.01 EU/ug).
- Safety Assurance: Sterile filtration through 0.2 μm membrane.
- AOF: Animal origin-free raw materials throughout the production process.
Source
Monoclonal Anti-CD16 Antibody, Mouse IgG1 (Clone: 3G8), Research Grade (CD6-CH29GN) is recombinantly produced from CHO cells.
Isotype
Mouse IgG1 | Mouse kappa
Conjugate
Unconjugated
Specificity
This product is a specific antibody that specifically reacts with CD16/Fc gamma RIII.
Endotoxin
Less than 0.002 EU per μg by the LAL method / rFC method.
Purity
>95% as determined by SDS-PAGE.
Formulation
Supplied as 0.2 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Shipping
This product is supplied and shipped with dry ice, please inquire the shipping cost.
Storage
For long term storage, the product should be stored at liquid state at -70°C or below.
This product is stable after storage at:
- -70°C for 3 years;
- -20°C for 12 months under sterile conditions;
- 2-8°C for 6 months under sterile conditions.
ACRO Quality Management System
Quality Description
ACRO's Research-grade products are suitable for a wide range of cell culture applications, particularly for research use in academic institutions. These products are sterilized by filtration, followed by lyophilization where applicable. Typical specifications include endotoxin levels of <0.01 EU/μg and purities >95%. Biological activity is calibrated against WHO/NIBSC standards when available.
ACRO's Premium-grade (Pre-GMP) products are characterized by their high quality and enhanced safety profiles, making them ideal for early-stage discovery and manufacturing processes in cell therapy companies. A key advantage is their seamless transition to corresponding GMP-grade versions. Biological activity is calibrated against WHO/NIBSC standards when available. Typical specifications include endotoxin levels of <0.01 EU/μg and purities >95%. In addition, rigorous testing is conducted to ensure the absence of mycoplasma, HCD, and HCP, thereby guaranteeing product safety.
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Performance Data
SDS-PAGE

Monoclonal Anti-CD16 Antibody, Mouse IgG1 (Clone: 3G8), Research Grade on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
Bioactivity-CELL BASE

Monoclonal Anti-CD16 Antibody, Mouse IgG1 (Clone: 3G8), Research Grade (Cat. No. CD6-CH29GN) stimulates Human CD16a (158V) (Luc) Jurkat Reporter Cell. The typical EC50 for this effect is 126.4 ng/mL (QC tested).
Protocol
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