Monoclonal Anti-Rabies virus Nucleoprotein Antibody, Mouse IgG1 (10B6) (MALS verified)

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Cat. No. / Size
Price
Qty
NUN-MY311-100ug
$360.00
NUN-MY311-1mg
$2380.00
ETA of in-stock products:2 business days

Product Details

  • Source

    Monoclonal Anti-Rabies virus Nucleoprotein Antibody, Mouse IgG1 (10B6) is a Mouse monoclonal antibody recombinantly expressed from HEK293 cells.

  • Clone

    10B6

  • Species

    Mouse

  • Isotype

    Mouse IgG1 | Mouse Kappa

  • Conjugate

    Unconjugated

  • Antibody Type

    Recombinant Monoclonal

  • Immunogen

    Recombinant Rabies virus (strain ERA) Nucleoprotein is expressed from human HEK293 cells

  • Specificity

    Specifically recognizes RABV Nucleoprotein Protein.

  • Application

    Application
    Recommended Usage
    ELISA
    0.2-63 ng/mL
  • Purity

    95% as determined by SDS-PAGE.

    90% as determined by SEC-MALS.

  • Purification

    Protein A purified / Protein G purified

  • Formulation

    Lyophilized from a 0.22 μm-filtered solution in PBS (pH 7.4), with trehalose as protectant.

    Please contact us for customized product forms or formulations.

  • Reconstitution

    Please refer to the Certificate of Analysis (CoA) for specific instructions.

    For best performance, we strongly recommend following the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored in a lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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Performance Data

  • SDS-PAGE

    Nucleoprotein SDS-PAGE

    Monoclonal Anti-Rabies virus Nucleoprotein Antibody, Mouse IgG1 (10B6) on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).

  • SEC-MALS

    Nucleoprotein SEC-MALS

    The purity of Monoclonal Anti-Rabies virus Nucleoprotein Antibody, Mouse IgG1 (10B6) (Cat. No. NUN-MY311) is more than 90% and the molecular weight of this protein is around 135-165 kDa verified by SEC-MALS.

    Report
  • Bioactivity-ELISA

     Nucleoprotein ELISA

    Immobilized Rabies virus (strain ERA) Nucleoprotein, His Tag (Cat. No. NUN-R55H3) at 1 μg/mL (100 μL/well) can bind Monoclonal Anti-Rabies virus Nucleoprotein Antibody, Mouse IgG1 (10B6) (Cat. No. NUN-MY311) with a linear range of 0.2-8 ng/mL (QC tested).

    Protocol

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Background

Rabies virus (RABV), scientific name Rabies lyssavirus, is a deadly neurotropic virus that causes rabies in humans and animals. Rabies virus has an extremely wide host range and its transmission most often occur through the saliva of animals. Without intervention prior to disease progression, rabies has the highest case fatality of any infectious disease. RABV contains a single-stranded negative-sense RNA genome that encodes five structural proteins: nucleoprotein (N), phosphoprotein (P), matrix protein (M), glycoprotein (G), and RNA-dependent RNA polymerase (L). Among these viral proteins, the RABV glycoprotein (RABV-G) is a pivotal player mediating virus entry and the major target of neutralizing antibodies, thus a key factor for vaccine and drug design.

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