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Product Overview
This Sample Dilution Buffer is designed for sample dilution in TR-FRET Assay, and is particularly well suited when Europium cryptate is involved in the TR-FRET Assay.
Storage
1. Store the unopened buffer at 2-8℃ upon receipt.
2. Locate the expiration date on the outer packaging and do not use reagents beyond their expiration date.
3. The shelf life of the opened buffer is 30 days from the date of opening.
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Performance Data
Bioactivity-TR-FRET
Please refer to DS document for the assay protocol.
Inhibition of Europium-chelate labeled human TL1A: FA labeled human DR3 binding by Human Anti-TL1A Neutralizing Antibody
Premix serial dilutions of Human Anti-TL1A Neutralizing Antibody (1:2 serial dilution, from 10 μg/mL to 0.01953125 μg/mL (66.6667-0.1302 nM)) and Human TL1A Protein Europi-um-chelate and incubate at room temperature (20℃-25℃) for 0.5 hours. Then add FA La-beled Human DR3 Protein and incubate at room temperature (20℃-25℃) for 0.5 hours. Detection was performed with IC50 of 2.816nM. The assay was performed according to the above-described Datasheet (QC tested).
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