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Product Details
Product Overview
Activin E is a key member of the transforming growth factor-β (TGF-β) superfamily. Primarily encoded by the INHBE gene and highly expressed in the liver, it is a dimeric protein (≈23 kDa) formed by two βE subunits linked via disulfide bonds. This kit is specifically designed for the accurate quantitation of mature human Activin E Dimer from cell culture supernates, serum and plasma. The standard is a recombinant mature Activin E dimer protein, and the antibodies specifically recognize the mature Activin E dimer. Our ELISA Kit provides several benefits:
- Fully validation in biologic samples for detection range, sensitivity, inter- and intra-plate CV, recovery, dilution linearity, specificity, and matrix effects to ensure reliable results according to ICH M10 guideline.
- High-quality antibody pairs and protein standards, along with rigorous quality control, to guarantee consistent results across different batches.
- Simplified and straightforward protocols and ready-to-use reagents to save assay time.
Product Specifications
Assay TypeSandwich-ELISAAnalyteActivin E DimerFormat96-wells plate breakable into 12 x 8 wells stripsReactivityHumanSensitivity60 pg/mLAssay Time2.75 hrSample volume50 μLRange78.125 pg/mL-5000 pg/mLSample TypeFor the quantitative determination human Activin E Dimer in serum, plasma and cell supernatant.Elevate your research experience with our Cytokine/Biomarker Detection Kits, where accuracy, reliability, and ease of use are converging to deliver exceptional results.
Application
The kit is developed for quantitative detection of natural and recombinant mature human Activin E dimer (not monomeric INHBE) in serum, plasma and cell culture supernates.
It is for research use only.
Workflow
Storage
Keep the unopened kit stored at 2-8 °C. Avoid using the kit beyond its expiration date. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
Materials Provided
IDComponentsSizeCEA247-C01Pre-coated Anti-Activin E Dimer Antibody Microplate1 plateCEA247-C02Human Activin E Dimer Standard1.8 μg×2CEA247-C03Biotin-Anti-Activin E Dimer Antibody Con. Solution100 μLCEA247-C04Biotin-Antibody Dilution Buffer8 mLCEA247-C05Streptavidin-HRP Con. Solution500 μLCEA247-C06Streptavidin-HRP Dilution Buffer15 mLCEA247-C0720×Washing Buffer50 mLCEA247-C08Sample Dilution Buffer15 mL×2CEA247-C09Substrate Solution12 mLCEA247-C10Stop Solution6 mLACRO Quality Management System
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Performance Data
Typical Data
Please refer to DS document for the assay protocol.
For each experiment, a standard curve needs to be set for each microplate, and the specific OD value may vary depending on different laboratories, testers, or equipment. The following example data is for reference only. The sample concentration was calculated based on the results of the standard curve. The minimum detectable concentration of Human Activin E is less than 60 pg/mL.
Sample Values
Healthy and High BMI donor serum samples were evaluated for the concentrations of human Activin E in assay. The sample detection rate was 100%. Mean of healthy donor is 12.78 ng/mL and mean of high BMI donor is 28.13 ng/mL.
Methodology Comparison
Comparison of LC-MS/MS and ELISA for the quantification of Activin E in human serum: Excellent correlation and high result consistency between the two detection methods.
Cross Verification
Activin E (Oligomeric/non-dimeric forms) and Activin C were detected in this assay. The cross-reactivity rate (%) is shown in the following Table.
Intra-Assay Statistics
Ten replicates of each of three samples containing different Activin E concentrations were tested in one assay. Acceptable criteria: CV＜10%.
Inter-Assay Statistics
Three samples containing different concentrations of Activin E were tested in independent assays. Acceptable criteria: CV<15%.
Recovery
Recombinant Activin E was spiked into three diluted human serum samples, and then analyzed. The average recovery of Activin E for serum samples is 91.1%.
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