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Product Details
Product Specifications
Assay TypeSandwich-ELISAReactivityStreptococcus pyogenesAnalyteCas9Regulatory StatusRUOFormat96T (8×12 strips)Assay Time3 hr 20 minSample volume100 μLSensitivity＜0.781 ng/mLStandard Curve Range0.781 ng/mL-50 ng/mLSuitable Sample TypeFor the quantitative determination of Cas9 in Cell Therapy.Product Overview
Cas9 (CRISPR Associated Protein 9) ELISA Kit (Residue Testing) is based on the Sandwich-ELISA method and is used for the quantitative detection of Cas9 residues in cell therapy products. It is designed to provide a reliable tool for supporting quality control in gene and cell therapy manufacturing by monitoring Cas9 residues during in-process testing and final product release, ensuring safety, and compliance in CRISPR-based therapeutic applications.
Reconstitution
Please see Certificate of Analysis for details of reconstitution instruction and specific concentration.
Storage
1. Unopened kit should be stored at 2℃-8℃ upon receiving.
2. Find the expiration date on the outside packaging and do not use reagents past their expiration date.
3. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
Materials Provided
IDComponentsSizeCAS01-C01Pre-coated Anti-Cas9 Antibody Microplate1 plate (8×12 strips)CAS01-C02Cas9 Nuclease Standard20 μgCAS01-C03Biotin-Anti-Cas9 Antibody15 μgCAS01-C04Streptavidin-HRP50 μLCAS01-C0510xWashing Buffer50 mLCAS01-C062xDilution Buffer50 mLCAS01-C07Substrate Solution12 mLCAS01-C08Stop Solution7 mLACRO Quality Management System
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Performance Data
Typical Data
Please refer to DS document for the assay protocol.
For each experiment, a standard curve needs to be set for each micro-plate, and the specific OD value may vary depending on different laboratories, testers, or equipments. The following example data is for reference only.
Dilution Linearity
To assess the linearity of the assay, samples spiked with high concentrations of Cas9 nuclease were serially diluted with calibrator diluent to produce samples with values within the dynamic range of the assay.
Intra-Assay Statistics
Three samples of known concentration were tested ten times on one plate to assess intra-assay precision.
Inter-Assay Statistics
Three samples of known concentration were tested in three separate assays to assess inter-assay precision.
Recovery
Three Cas9 nuclease with different concentrations were tested to calculate the recovery rate.
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