Product Details
Product Specifications
Assay TypeSandwich-ELISAAnalyteFGF2Format96T (8×12 strips)ReactivityHumanRegulatory StatusRUOSensitivity<15.625 pg/mLStandard Curve Range15.625 pg/mL-500 pg/mLAssay Time2 hr 50 minSuitable Sample TypeFor the quantitative determination of human FGF2 in Cell Culture Supernatants, Plasma, Serum.Sample volume100 μLProduct Overview
Human FGF2 ELISA Kit (Residue Testing) is based on the ELISA sandwich method and is used to detect and quantitatively determine GMP human FGF2 residues in cell culture supernatants, serum, and plasma. The kit contains GMP human FGF2 (ACROBiosystems, cat# GMP-FGCH17) to ensure accurate assay results and is designed to provide a reliable solution for CAR-T product quality assessment during drug development and CMC quality control stages. It can also be used as a universal detection tool for the quantitative determination of human FGF2
Reconstitution
Please see Certificate of Analysis for details of reconstitution instruction and specific concentration.
Storage
1. Unopened kit should be stored at 2℃-8℃ upon receiving.
2. Find the expiration date on the outside packaging and do not use reagents past their expiration date.
3. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
Materials Provided
IDComponentsSizeRES009-C01Pre-coated Anti-FGF2 Antibody Microplate1 plate (8×12 strips)RES009-C02Human FGF2 Standard15 μgRES009-C03Biotin-Anti-FGF2 Antibody20 μgRES009-C04Streptavidin-HRP50 μLRES009-C0510×Washing Buffer50 mLRES009-C062×Dilution Buffer50 mLRES009-C07Substrate Solution12 mLRES009-C08Stop Solution7 mLACRO Quality Management System
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Performance Data
Typical Data
Please refer to DS document for the assay protocol.

For each experiment, a standard curve needs to be set for each micro-plate, and the specific OD value may vary depending on different laboratories, testers, or equipments. The following example data is for reference only.
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