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Product Details
Product Overview
Human IL-2 Detection Kit (TR-FRET) can detect the human IL-2 in homogeneous system within 2 hours, it is highly sensitive, short detection time, easy to use and possesses high-throughput screening capability. Compatible with microplate formats, this kit supports high-throughput screening workflows, enabling efficient large-scale sample analysis. This kit is specifically designed for the accurate quantitation of human IL-2 from cell culture supernatant, serum and plasma.
- Simplified and straightforward protocols and ready-to-use reagents to save assay time.
- Standards to calibrate with NIBSC/WHO standards for comparable results.
- Fully validation in biologic samples for detection range, sensitivity, inter- and intra-plate CV, recovery, dilution linearity, specificity, and matrix effects to ensure reliable results according to ICH M10 guideline.
- High-quality antibody pairs and protein standards, along with rigorous quality control, to guarantee consistent results across different batches.
Product Specifications
Assay TypeSandwich-TR-FRETAnalyteHuman IL-2Format100T/500TReactivityHumanSensitivityLOQ=62.5pg/mLRange62.5 pg/mL-8000 pg/mLAssay Time2 hrSample volume16 μLSample TypeFor human TNF-α detection in human serum, human plasma,cell culture supernatants.Elevate your research experience with our Cytokine/Biomarker Detection Kits, where accuracy, reliability, and ease of use are converging to deliver exceptional results.
Application
The kit is developed for quantitative detection of human IL-2 in serum and cell culture supernates.
It is for research use only.
Workflow
Storage
Keep the unopened kit stored at 2-8 °C. Avoid using the kit beyond its expiration date. The opened kit should be stored per components table.
Materials Provided
IDComponentsSizeFRTC03-C01Anti-Human IL-2 Antibody Europium-chelate100 tests/500 testsFRTC03-C02FA Labeled Anti-Human IL-2 Antibody100 tests/500 testsFRTC03-C03Human IL-2 Standard15 μg/100 tests & 500 testsDB-04TR-FRET Sample Dilution Buffer, pH7.450 mL/100 tests & 500 testsDB-05TR-FRET Detection Buffer, pH7.450 mL/100 tests & 500 testsACRO Quality Management System
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Performance Data
Typical Data
Please refer to DS document for the assay protocol.
Standard curve generated in TR-FRET Sample Dilution Buffer, pH7.4 (DB-04).
Dilution Linearity
To evaluate the linearity of the assay, samples spiked with high-concentration human IL-2 in various matrices were prepared. With DB-04 (TR-FRET Sample Dilution Buffer, pH 7.4) as the surrogate matrix, standard curves were generated and the spiked samples were subjected to serial gradient dilution, followed by the quantification of the spiked samples.
Intra-Assay Statistics
Five samples with known concentrations were tested repeatedly 16 times within a single analytical batch to assess within-batch precision and accuracy.
Inter-Assay Statistics
Five samples with known concentrations were tested repeatedly across three independent analytical batches to evaluate between-batch precision and accuracy.
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Recent Advances
Drug Development Progress- English Name：
Interleukin-2
- Category：
- Approved Drugs：
2 Details
- Drugs in Clinical Trials：
46 Details
- Highest Development Stage：
Approved
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