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Product Details
Product Specifications
Assay TypeSandwich-ELISAAnalyteTPOFormat96T (8×12 strips)ReactivityHumanRegulatory StatusRUOSensitivity＜12.5 pg/mLStandard Curve Range12.5 pg/mL-400 pg/mLAssay Time3 hr 20 minSuitable Sample TypeFor the detection and quantitative determination of TPO in human serum and cell culture supernates.Sample volume100 μLProduct Overview
Human TPO ELISA Kit (Residue Testing) is based on the ELISA sandwich method and is used to detect and quantitatively determine GMP Human TPO residues in cell culture supernatants, serum, and plasma. The kit contains GMP human TPO (ACROBiosystems, cat# GMP-THNH25) to ensure accurate assay results and is designed to provide a reliable solution for CAR-T product quality assessment during drug development and CMC quality control stages. It can also be used as a universal detection tool for the quantitative determination of Human TPO.
Reconstitution
Please see Certificate of Analysis for details of reconstitution instruction and specific concentration.
Storage
1. Unopened kit should be stored at 2℃-8℃ upon receiving.
2. Find the expiration date on the outside packaging and do not use reagents past their expiration date.
3. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
Materials Provided
IDComponentsSizeRES034-C01Pre-coated Anti-TPO Antibody Microplate1 plate (8×12 strips)RES034-C02Human TPO Standard20 μgRES034-C03Biotin-Anti-TPO Antibody20 μgRES034-C04Streptavidin-HRP50 μLRES034-C0510×Washing Buffer50 mLRES034-C062×Dilution Buffer50 mLRES034-C07Substrate Solution12 mLRES034-C08Stop Solution7 mLACRO Quality Management System
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Performance Data
Typical Data
Please refer to DS document for the assay protocol.
For each experiment, a standard curve needs to be set for each micro-plate, and the specific OD value may vary depending on different laboratories, testers, or equipments. The following example data is for reference only.
Dilution Linearity
To assess the linearity of the assay, samples spiked with high concentrations of TPO were serially diluted with calibrator diluent to produce samples with values within the dynamic range of the assay.
Intra-Assay Statistics
Three samples of known concentration were tested ten times on one plate to assess intra-assay precision.
Inter-Assay Statistics
Three samples of known concentration were tested in three separate assays to assess inter-assay precision.
Recovery
Five parts of blank T cell culture supernatant were added with different concentrations of human TPO, and the T cell culture supernatant without human TPO was used as background to calculate the recovery rate. The range of the recovery rate is 94.7-108.9%, and the average recovery is 102.1%.
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