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Product Details
Synonym
TNFRSF9, 4-1BB, CD137, CDw137, ILA
Source
Biotinylated Human 4-1BB, His,Avitag (41B-H82E6) is expressed from human 293 cells (HEK293). It contains AA Leu 24 - Gln 186 (Accession # Q07011-1).
Predicted N-terminus: Leu 24
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).
The protein has a calculated MW of 20.9 kDa. The protein migrates as 30-35 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Labeling
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
Protein Ratio
Passed as determined by the HABA assay / binding ELISA.
Purity
>90% as determined by SDS-PAGE.
>95% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE
Biotinylated Human 4-1BB, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Biotinylated Human 4-1BB, His,Avitag (Cat. No. 41B-H82E6) is more than 95% and the molecular weight of this protein is around 25-35 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA
Immobilized UreluMab, Human IgG4 at 1 μg/mL (100 μL/well) can bind Biotinylated Human 4-1BB, His,Avitag (Cat. No. 41B-H82E6) with a linear range of 1-15 ng/mL (QC tested).
Protocol
Immobilized Biotinylated Human 4-1BB, His,Avitag (Cat. No. 41B-H82E6) at 1 μg/mL (100 μL/well) on Recombinant Streptavidin (Cat. No. STN-N5116) precoated (0.5μg/well) plate can bind Human 4-1BB Ligand, Fc Tag with a linear range of 0.1-2 ng/mL (Routinely tested).
Protocol
Immobilized Utomilumab at 1 μg/mL (100 μL/well) can bind Biotinylated Human 4-1BB, His,Avitag (Cat. No. 41B-H82E6) with a linear range of 0.5-8 ng/mL (Routinely tested).
Protocol
Bioactivity-SPR
Biotinylated Human 4-1BB Protein, His,Avitag (Cat. No. 41B-H82E6) captured on Biotin CAP-Series S sensor Chip can bind anti 4-1BB mAb with an affinity constant of 0.158 nM as determined in a SPR assay (Biacore T200) (Routinely tested) .
Protocol
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Background
Recent Advances
Drug Development Progress- English Name：
Tumor necrosis factor receptor superfamily member 9
- Category：
- Approved Drugs：
0 Details
- Drugs in Clinical Trials：
77 Details
- Highest Development Stage：
Phase 3 Clinical
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