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Product Details
Synonym
Claudin-6, CLDN6
Source
Fluorescent Human Claudin-6 Protein, Flag,His,GFP Tag (CL6-HF2M9) is expressed from human 293 cells (HEK293). It contains AA Ala 2 - Val 220 (Accession # P56747).
Predicted N-terminus: Met
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a flag tag at the N-terminus, followed by a polyhistidine tag, and a GFP tag at the C-terminus.
The protein has a calculated MW of 54.0 kDa. The protein migrates as 22 kDa, 25 kDa, 51-52 kDa and 100 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Nanodiscs are a new class of model membranes that are being used to solubilize and study a range of integral membrane proteins and membrane-associated proteins. The Nanodisc bilayer is bounded by an amphipathic scaffold coat that confers enhanced stability and a narrow particle size distribution.
The nanodisc assembles from a mixture of full length membrane protein in detergent, phospholipid micelles and membrane scaffold protein upon removal of the detergent.
Conjugate
GFP
Excitation source: 488 nm spectral line, argon-ion laser
Excitation Wavelength: 488 nm
Emission Wavelength: 530 nm
Purity
>80% as determined by SDS-PAGE.
Formulation
Supplied as 0.2 μm filtered solution in 20 mM HEPES, 150 mM NaCl, pH7.5 with trehalose as protectant.
Contact us for customized product form or formulation.
Shipping
This product is supplied and shipped with dry ice, please inquire the shipping cost.
Storage
Please protect from light and avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- The product MUST be stored at -70°C or lower upon receipt;
- -70°C for 3 months under sterile conditions.
ACRO Quality Management System
- *The isotype control of empty/mock nanodisc (Cat. No. APO-H51H3) is sold separately and not included in protein, you can follow this link for product information.
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Performance Data
SDS-PAGE
Fluorescent Human Claudin-6 Protein, Flag,His,GFP Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 80%.
Bioactivity-ELISA
Immobilized Fluorescent Human Claudin-6 Protein, Flag,His,GFP Tag (Cat. No. CL6-HF2M9) at 5 μg/mL (100 μL/well) can bind Monoclonal Anti-Human Claudin 6 antibody, Human IgG1 with a linear range of 0.1-3 ng/mL (QC tested).
Protocol
Bioactivity-SPR
Anti-Claudin-6 Antibody, Human IgG1 captured on Protein A Chip can bind Fluorescent Human Claudin-6 Protein, Flag,His,GFP Tag (Cat. No. CL6-HF2M9) with an affinity constant of 34.1 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
Evaluation of CAR expression
FACS Analysis of Anti-Claudin-6 CAR Expression
2e5 of anti-Claudin-6 CAR-293 cells were stained with 100 μL of 1 μg/mL of Fluorescent Human Claudin-6 Protein, Flag,His,GFP Tag (Cat. No.CL6-HF2M9) and negative control protein respectively (Fig. A and B), FITC signal was used to evaluate the binding activity (QC tested).
ProtocolFACS Analysis of Non-specific binding to PBMCs
5e5 of PBMCs were stained with Fluorescent Human Claudin-6 Protein, Flag,His,GFP Tag (Cat. No. CL6-HF2M9) and anti-CD3 antibody, washed and then analyzed with FACS. PE signal was used to evaluate the expression of CD3+ T cells in PBMCs, and FITC signal was used to evaluate the non-specific binding activity to PBMCs (QC tested).
Protocol
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Background
Recent Advances
Drug Development Progress- English Name：
Claudin 6
- Category：
- Approved Drugs：
0 Details
- Drugs in Clinical Trials：
15 Details
- Highest Development Stage：
Phase 2 Clinical
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