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Product Details
Synonym
EGFR, ERBB, ERBB1, HER1, PIG61, mENA
Source
Human EGF R, Llama IgG2b Fc Tag (EGR-H5259) is expressed from human 293 cells (HEK293). It contains AA Leu 25 - Ser 645 (Accession # P00533-1).
Predicted N-terminus: Leu 25
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a llama IgG2b Fc tag at the C-terminus.
The protein has a calculated MW of 96.4 kDa. The protein migrates as 115-150 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 0.01 EU per μg by the LAL method / rFC method.
Purity
>95% as determined by SDS-PAGE.
Formulation
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 25 mM Arginine, 150 mM NaCl, pH7.5 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
ACRO Quality Management System
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Performance Data
SDS-PAGE
Human EGF R, Llama IgG2b Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
SEC-MALS
The purity of Human EGF R, Llama IgG2b Fc Tag (Cat. No. EGR-H5259) is more than 85% and the molecular weight of this protein is around 220-265 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA
Immobilized Human EGF, His Tag (Cat. No. EGF-H52H3) at 5 μg/mL (100 μL/well) can bind Human EGF R, Llama IgG2b Fc Tag (Cat. No. EGR-H5259) with a linear range of 0.2-5 ng/mL (QC tested).
Protocol
Immobilized Human EGF R, Llama IgG2b Fc Tag (Cat. No. EGR-H5259) at 2 μg/mL (100 μL/well) can bind Cetuximab with a linear range of 0.3-5 ng/mL (Routinely tested).
Protocol
Immobilized Human EGF R, Llama IgG2b Fc Tag (Cat. No. EGR-H5259) at 1 μg/mL (100 μL/well) can bind Anti-EGFR Peptide with a linear range of 0.06-2 ng/mL (Routinely tested).
Protocol
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Background
Recent Advances
Drug Development Progress- English Name：
Epidermal growth factor receptor erbB1
- Category：
- Approved Drugs：
39 Details
- Drugs in Clinical Trials：
206 Details
- Highest Development Stage：
Approved
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