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Product Details
Features and Advantages
- Engineered via a Closed-Loop AI Design Platform – Enables rational sequence optimization to achieve maximal functional performance.
- Protected by PCT Patent – Guarantees intellectual property security and a reliable long-term supply.
- Superior Stability at 37°C for 3 Days – Provides unmatched operational flexibility and experimental convenience in cell culture workflows.
- Enables "Weekend-Free" Continuous Culture – Reduces manual handling, lowers contamination risk, and significantly cuts labor and reagent costs.
Synonym
FGF2, BFGF, FGFB, FGF basic, HBGF-2
Product Details
Our Recombinant Human FGF basic Superior Stable Mutant Protein, developed via an AI-modified and experimentally validated closed-loop efficient protein design platform, is the optimal FGF2 mutant with superior thermal stability and activity compared to wild-type FGF2, making it more suitable for various stem cell culture processes. This reduces medium replacement frequency, lowers contamination risks from frequent operations, and saves costs/time for stem cell culture by minimizing reagent consumption and labor, especially in large-scale applications.
This product is designed and developed utilizing our proprietary AI technology, which is protected by patent rights. We retain full commercial rights to this product.Molecular Characterization
This protein carries no "tag".
The protein has a calculated MW of 16.5 kDa. The protein migrates as 17 kDa±3 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE).
Endotoxin
Less than 0.01 EU per μg by the LAL method / rFC method.
Purity
>95% as determined by SDS-PAGE.
>95% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 24 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
ACRO Quality Management System
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Performance Data
SDS-PAGE
Human FGF basic Superior Stable Mutant Protein, Research Grade on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Human FGF basic Superior Stable Mutant Protein, Research Grade (Cat. No. BFF-H5114) is more than 95% and the molecular weight of this protein is around 13-21 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA
Immobilized Human FGF basic Superior Stable Mutant Protein, Research Grade (Cat. No. BFF-H5114) at 2 μg/mL (100 μL/well) can bind Human Glypican 3 Protein, Fc Tag (Cat. No. GP3-H5258) with a linear range of 2-25 ng/mL (QC tested).
Protocol
Bioactivity-CELL BASE
Human FGF basic Superior Stable Mutant Protein, Research Grade (Cat. No. BFF-H5113) stimulates proliferation of NIH-3T3 cells. The specific activity of Human FGF basic Superior Stable Mutant Protein, Research Grade > 1.60 x 10^7 IU/mg, which is calibrated against Basic Fibroblast Growth Factor WHO International Standard (NIBSC code: 90/712) (QC tested).
Protocol
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Background
Recent Advances
Drug Development Progress- English Name：
Fibroblast growth factor 2
- Category：
- Approved Drugs：
1 Details
- Drugs in Clinical Trials：
4 Details
- Highest Development Stage：
Approved
Contact Us
[North America]: +1 800-810-0816
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[Asia & Pacific]: +86 400-682-2521
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