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Product Details
Synonym
Glycoprotein H & Glycoprotein L/gH & gL (HCMV)
Source
HHV-5 (strain AD169) Glycoprotein H&L, His Tag (GHL-H5243) is expressed from human 293 cells (HEK293). It contains AA Val 31 - Arg 278 & Arg 24 - Leu 720 (Accession # P12824 & P16832).
Predicted N-terminus: His
Molecular Characterization
This protein carries a polyhistidine tag at the N-terminus.
The protein has a calculated MW of 109.5 kDa. The protein migrates as 110-130 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
The protein is designed as a dimer.
Endotoxin
Less than 1.0 EU per μg by the LAL method / rFC method.
Purity
>90% as determined by SDS-PAGE.
>95% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE
HHV-5 (strain AD169) Glycoprotein H&L, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of HHV-5 (strain AD169) Glycoprotein H&L, His Tag (Cat. No. GHL-H5243) is more than 95% and the molecular weight of this protein is around 210-250 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA
Immobilized HHV-5 (strain AD169) Glycoprotein H&L, His Tag (Cat. No. GHL-H5243) at 1 μg/mL (100 μL/well) can bind Anti-HCMV gH [HCMV16], Human, IgG1 with a linear range of 0.1-2 ng/mL (QC tested).
Protocol
Immobilized Anti-HCMV gH [HCMV16], Human, IgG1 at 1 μg/mL (100 μL/well) can bind HHV-5 (strain AD169) Glycoprotein H&L, His Tag (Cat. No. GHL-H5243) with a linear range of 0.004-0.25 μg/mL (Routinely tested).
Protocol
Bioactivity-SPR
Anti-HCMV gH [HCMV16] captured on Protein A Chip can bind HHV-5 (strain AD169) Glycoprotein H&L, His Tag (Cat. No. GHL-H5243) with an affinity constant of 1.49 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
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