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Product Details
Synonym
Glycoprotein H & Glycoprotein L & Glycoprotein O/gH & gL & gO (HCMV)
Source
Human cytomegalovirus (strain AD169) (HHV-5) Glycoprotein H&L&O, His Tag (GLO-V5243) is expressed from human 293 cells (HEK293). It contains AA Lys 31 - Gln 466 & Val 31 - Arg 278 & Arg 24 - Leu 720 (Accession # P12824 & P16832 & P16750).
Predicted N-terminus: His
Molecular Characterization
This protein carries a polyhistidine tag at the N-terminus.
The protein has a calculated MW of 162.2 kDa. The protein migrates as 180 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 1.0 EU per μg by the LAL method / rFC method.
Purity
>90% as determined by SDS-PAGE.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE
Human cytomegalovirus (strain AD169) (HHV-5) Glycoprotein H&L&O, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Human cytomegalovirus (strain AD169) (HHV-5) Glycoprotein H&L&O, His Tag (Cat. No. GLO-V5243) is more than 85% and the molecular weight of this protein is around 195-235 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA
Immobilized Human cytomegalovirus (strain AD169) (HHV-5) Glycoprotein H&L&O, His Tag (Cat. No. GLO-V5243) at 1 μg/mL (100 μL/well) can bind Anti-HCMV gH [HCMV16], Human, IgG1 with a linear range of 0.05-2 ng/mL (QC tested).
Protocol
Bioactivity-SPR
Human cytomegalovirus (strain AD169) (HHV-5) Glycoprotein H&L&O, His Tag (Cat. No. GLO-V5243) immobilized on CM5 Chip can bind Human PDGF R alpha, His Tag (Cat. No. PDA-H52H7) with an affinity constant of 2.52 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
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