Product Details
Synonym
Hemagglutinin 1/HA 1 (Influenza Virus)
Source
Influenza A [A/guinea fowl/Hong Kong/WF10/99(H9N2)] HA1 Protein, His Tag (HA1-V52H5) is expressed from human 293 cells (HEK293). It contains AA Asp 19 - Arg 338 (Accession # Q80KD9_9INFA).
Predicted N-terminus: Asp 19
Request for sequenceMolecular Characterization

Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 40.9 kDa. The protein migrates as 55-65 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
The protein is designed as a trimer.
Endotoxin
Less than 1.0 EU per μg by the LAL method / rFC method.
Purity
>90% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in 0.1 M Sodium citrate, pH5.5 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE

Influenza A [A/guinea fowl/Hong Kong/WF10/99(H9N2)] HA1 Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.
SEC-MALS

The purity of Influenza A [A/guinea fowl/Hong Kong/WF10/99(H9N2)] HA1 Protein, His Tag (Cat. No. HA1-V52H5) is more than 90% and the molecular weight of this protein is around 170-190 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA

Immobilized Influenza A [A/guinea fowl/Hong Kong/WF10/99(H9N2)] Hemagglutinin 1 (HA1) Protein, His Tag (Cat. No. HA1-V52H5) at 1 μg/mL (100 μL/well) can bind Anti-HA Monoclonal Antibody (H9N2) with a linear range of 0.1-4 ng/mL (QC tested).
Protocol
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