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Product Details
Synonym
MET, AUTS9, HGFR, RCCP2, c-Met
Source
Cynomolgus HGF R, His Tag (MET-C52H9) is expressed from human 293 cells (HEK293). It contains AA Glu 25 - Asn 930 (Accession # A0A2K5UM36-1).
Predicted N-terminus: Glu 25
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 103.2 kDa. The protein migrates as 80 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE).
Endotoxin
Less than 0.01 EU per μg by the LAL method / rFC method.
Purity
>90% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, 0.2 M Arginine, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE
Cynomolgus HGF R, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Cynomolgus HGF R, His Tag (Cat. No. MET-C52H9) is more than 90% and the molecular weight of this protein is around 110-140 kDa verified by SEC-MALS.
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Bioactivity-ELISA
Immobilized Cynomolgus HGF R, His Tag (Cat. No. MET-C52H9) at 5 μg/mL (100 μL/well) can bind Human HGF, Fc Tag (Cat. No. HGF-H5253) with a linear range of 0.6-20 ng/mL (QC tested).
Protocol
Immobilized Human HGF, Fc Tag (Cat. No. HGF-H5253) at 2 μg/mL (100 μL/well) can bind Cynomolgus HGF R, His Tag (Cat. No. MET-C52H9) with a linear range of 0.039-1.25 μg/mL (Routinely tested).
Protocol
Bridging ELISA
Validated with Bispecific Antibody.
Immobilized Rhesus macaque EGF R Protein, Fc Tag (Cat. No. EGR-C5252) at 10 μg/mL, add increasing concentrations of Amivantamab and then add Cynomolgus HGF R Protein, His Tag (Cat. No. MET-C52H9) at 5 μg/mL. Detection was performed using HRP conjugated Anti-His Antibody (AY63), mAb (Cat. No. HIS-PLM535) with sensitivity of 0.02 μg/mL (Routinely tested).
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Background
Recent Advances
Drug Development Progress- English Name：
Hepatocyte growth factor receptor
- Category：
- Approved Drugs：
13 Details
- Drugs in Clinical Trials：
74 Details
- Highest Development Stage：
Approved
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