Save Product
Product Details
Synonym
MSLN, Mesothelin, MPF
Source
Biotinylated Human Mesothelin (296-580), His,Avitag, premium grade (MSN-H82E9) is expressed from human 293 cells (HEK293). It contains AA Glu 296 - Gly 580 (Accession # AAH09272.1).
Predicted N-terminus: Glu 296
It is produced under our rigorous quality control system that incorporates a comprehensive set of tests including sterility and endotoxin tests. Product performance is carefully validated and tested for compatibility for cell culture use or any other applications in the early preclinical stage.
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).
The protein has a calculated MW of 35.9 kDa. The protein migrates as 40-50 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Labeling
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
Protein Ratio
Passed as determined by the HABA assay / binding ELISA.
Endotoxin
Less than 0.01 EU per μg by the LAL method / rFC method.
Sterility
Negative
Mycoplasma
Negative
Purity
>95% as determined by SDS-PAGE.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
ACRO Quality Management System
Customers Also Viewed
Performance Data
SDS-PAGE
Biotinylated Human Mesothelin (296-580), His,Avitag, premium grade on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Biotinylated Human Mesothelin (296-580), His,Avitag, premium grade (Cat. No. MSN-H82E9) is more than 85% and the molecular weight of this protein is around 38-53 kDa verified by SEC-MALS.
Report
Bioactivity-FACS
2e5 of Anti-MSLN CAR-293 cells were stained with 100μL of 1 μg/mL of Biotinylated Human Mesothelin (296-580), His,Avitag, premium grade (Cat. No. MSN-H82E9) and negative control protein respectively, washed and then followed by PE-SA and analyzed with FACS (QC tested).
Protocol
Bioactivity-ELISA
Immobilized Anti-Human MSLN MAb at 0.5 μg/mL (100 μL/well) can bind Biotinylated Human Mesothelin (296-580), His,Avitag, premium grade (Cat. No. MSN-H82E9) with a linear range of 0.1-0.78 ng/mL (QC tested).
Protocol
Evaluation of CAR expression
FACS Analysis of Anti-MSLN CAR Expression
293 cells were transfected with Anti-MSLN-scFv and RFP tag. 2e5 of the cells were first stained with B. Biotinylated Human Mesothelin (296-580), His,Avitag, premium grade (Cat. No. MSN-H82E9, 3 µg/mL) and C. Biotinylated Protein Control, followed by FITC Streptavidin. A. Non-transfected 293 cells and C. Biotinylated Protein Control were used as negative control. RFP was used to evaluate CAR (Anti-MSLN-scFv) expression and FITC was used to evaluate the binding activity of Biotinylated Human Mesothelin (296-580), His,Avitag, premium grade (Cat. No. MSN-H82E9).
Protocol
Customer Reviews Writing Reviews
Background
Recent Advances
Drug Development Progress- English Name：
Mesothelin
- Category：
- Approved Drugs：
0 Details
- Drugs in Clinical Trials：
55 Details
- Highest Development Stage：
Phase 2 Clinical
Contact Us
[North America]: +1 800-810-0816
[Switzerland]: +41 800 040 012
[Asia & Pacific]: +86 400-682-2521
Drug Candidate Licensing
