Human NCAM-1 / CD56 Protein, His Tag, low endotoxin (MALS verified)

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Cat. No. / Size
Price
Qty
NC1-H5223-100ug
$415.00
NC1-H5223-1mg (500ug X 2)
$2625.00
ETA of in-stock products:2 business days

Product Details

  • Synonym

    CD56, MSK39, NCAM1, N-CAM-1

  • Source

    Human NCAM-1, His Tag (NC1-H5223) is expressed from human 293 cells (HEK293). It contains AA Leu 20 - Gly 718 (Accession # P13591-2).

    Predicted N-terminus: Leu 20

    Request for sequence
  • Molecular Characterization

    NCAM-1 Structure

    Other Tags and Version Biotin & Other Labeled Version

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 79.3 kDa. The protein migrates as 100-120 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin

    Less than 0.01 EU per μg by the LAL method / rFC method.

  • Purity

    >95% as determined by SDS-PAGE.

    >95% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 500 mM NaCl, pH8.0 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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Performance Data

  • SDS-PAGE

    NCAM-1 SDS-PAGE

    Human NCAM-1, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).

  • SEC-MALS

    NCAM-1 SEC-MALS

    The purity of Human NCAM-1, His Tag (Cat. No. NC1-H5223) is more than 95% and the molecular weight of this protein is around 120-135 kDa verified by SEC-MALS.

    Report
  • Bioactivity-ELISA

     NCAM-1 ELISA

    Immobilized Human NCAM-1, His Tag (Cat. No. NC1-H5223) at 1 μg/mL (100 μL/well) can bind Anti-NCAM-1 Antibody, Human IgG1 with a linear range of 0.6-10 ng/mL (QC tested).

    Protocol

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Background

NCAM1 belongs to the immunoglobulin superfamily of adhesion molecules. A wide range of alternatively spliced NCAM1 messenger RNAs (mRNAs) has been described to date, but only the 120-, 140-, and 180- kDa isoforms are commonly expressed. NCAM1 plays an important role in the regulation of neurogenesis, neurite outgrowth, proliferation, and cell migration, however, its function in hematopoiesis, including NK cells, is poorly understood. NCAM1 signaling is mediated either by homophilic or heterophilic interactions with fibroblast growth factor receptor (FGFR), L1-CAM, N-cadherin and other components of the extracellular matrix. Upon activation, NCAM1 triggers a variety of signaling cascades including FYN–focal adhesion kinase (FAK), MAPK, and phosphatidylinositol 3-kinase (PI3K) pathways.

Recent Advances

 
Drug Development Progress
  • English Name:

    Neural cell adhesion molecule 1

  • Category:

  • Approved Drugs:

    0 Details

  • Drugs in Clinical Trials:

    5 Details

  • Highest Development Stage:

    Phase 2 Clinical

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