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Product Details
Synonym
Spike, S protein RBD, Spike glycoprotein Receptor-binding domain, S glycoprotein RBD, Spike protein RBD
Source
Biotinylated SARS-CoV-2 Spike RBD, His,Avitag (SPD-C82Eh) is expressed from human 293 cells (HEK293). It contains AA Arg 319 - Lys 537 (Accession # QHD43416.1 (L452Q, F490S)). Mutations L452Q, F490S were identified in the SARS-CoV-2 Lambda variant (Pango lineage: C.37).
Predicted N-terminus: Arg 319
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).
The protein has a calculated MW of 28.2 kDa. The protein migrates as 33-38 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Labeling
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
Protein Ratio
Passed as determined by the HABA assay / binding ELISA.
Purity
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE
Biotinylated SARS-CoV-2 Spike RBD (L452Q, F490S), His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
SEC-MALS
The purity of Biotinylated SARS-CoV-2 Spike RBD (L452Q, F490S), His,Avitag (Cat. No. SPD-C82Eh) is more than 90% and the molecular weight of this protein is around 27-41 kDa verified by SEC-MALS.
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Bioactivity-ELISA
Immobilized Biotinylated SARS-CoV-2 Spike RBD, His,Avitag (Cat. No. SPD-C82Eh) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Human ACE2, Fc Tag (Cat. No. AC2-H5257) with a linear range of 0.1-3 ng/mL (QC tested).
Protocol
Immobilized Biotinylated SARS-CoV-2 Spike RBD, His,Avitag (Cat. No. SPD-C82Eh) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Anti-SARS-CoV-2 Spike RBD Antibody, Chimeric mAb, Human IgG1 (Cat. No. S1N-M122) with a linear range of 0.1-2 ng/mL (Routinely tested).
Protocol
Immobilized Human ACE2, Fc Tag (Cat. No. AC2-H5257) at 5 μg/mL (100 μL/well) can bind Biotinylated SARS-CoV-2 Spike RBD, His,Avitag (Cat. No. SPD-C82Eh) with a linear range of 2-156 ng/mL (Routinely tested).
Protocol
Immobilized Anti-SARS-CoV-2 Spike RBD Antibody, Chimeric mAb, Human IgG1 (Cat. No. S1N-M122) at 1 μg/mL (100 μL/well) can bind Biotinylated SARS-CoV-2 Spike RBD, His,Avitag (Cat. No. SPD-C82Eh) with a linear range of 0.1-3 ng/mL (Routinely tested).
Protocol
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