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Product Details
Synonym
CD71, TFRC, TFR, sTfR, TfR1, Trfr, T9, p90
Source
Human Transferrin R Protein, Tag Free (TFR-H5213) is expressed from human 293 cells (HEK293). It contains AA Cys 89 - Phe 760 (Accession # P02786-1).
Predicted N-terminus: Cys 89
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries no "tag".
The protein has a calculated MW of 75.2 kDa. The protein migrates as 90-95 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
The protein is designed as a dimer.
Endotoxin
Less than 1.0 EU per μg by the LAL method / rFC method.
Purity
>95% as determined by SDS-PAGE.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE
Human Transferrin R Protein, Tag Free on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Human Transferrin R Protein, Tag Free (Cat. No. TFR-H5213) is more than 85% and the molecular weight of this protein is around 135-180 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA
Immobilized Human Transferrin R Protein, Tag Free (Cat. No. TFR-H5213) at 5 μg/mL (100 μL/well) can bind Biotinylated Human Transferrin Protein, His,Avitag (Cat. No. TRN-H82E3) with a linear range of 0.2-6 ng/mL (QC tested).
Protocol
Bioactivity-SPR
Monoclonal Anti-CD71 Antibody, Mouse IgG1 (5B5) (Cat. No. CD1-MY2101) captured on CM5 chip via anti-mouse antibodies surface can bind Human Transferrin R Protein, Tag Free (Cat. No. TFR-H5213) with an affinity constant of 0.570 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
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Background
Recent Advances
Drug Development Progress- English Name：
Transferrin receptor protein 1
- Category：
- Approved Drugs：
1 Details
- Drugs in Clinical Trials：
15 Details
- Highest Development Stage：
Approved
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