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FITC-Labeled Monoclonal Anti-Human CD19 Antibody, Mouse IgG2a (FMC63) (0.03% Proclin)

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  • 抗体来源(Source)

    Monoclonal Anti-Human CD19 Antibody, Mouse IgG2a (FMC63) is a mouse monoclonal antibody recombinantly expressed from human 293 cells (HEK293), which provides higher batch consistency and long term security of supply.

  • 应用(Application)

    Flow Cytometry (Detection the expression of CD19 on Human cells).

  • 克隆号(Clone)

    FMC63

  • 种属(Species)

    Mouse

  • 亚型(Isotype)

    Mouse IgG2a | Mouse kappa

  • 特异性(Specificity)

    This product is a specific antibody specifically reacts with CD19 protein.

  • 种属反应性(Reactivity)

    Human

  • 免疫原(Immunogen)

    Human prolymphocytic leukaemia cell line JVM3.

  • 偶联(Conjugate)

    FITC

    Excitation source: 488 nm spectral line, argon-ion laser

    Excitation Wavelength: 488 nm

    Emission Wavelength: 535 nm

  • 推荐稀释比(Recommended Dilution)

    1:50

  • 制剂(Formulation)

    Supplied as 0.2 μm filtered solution in PBS, 0.03% Proclin 300, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • 存储(Storage)

    Please protect from light and avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. The product MUST be stored at -70°C or lower upon receipt;
    2. -70°C for 12 months under sterile conditions.
  • 质量管理控制体系(QMS)

    1. 质量管理体系(ISO, GMP)
    2. 质量优势
    3. 质控流程

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  • 活性(Bioactivity)-FACS

     CD19 FACS

    Flow cytometric analysis of Human peripheral blood lymphocytes staining with FITC-Labeled Monoclonal Anti-Human CD19 Antibody, Mouse IgG2a (FMC63) (Cat. No. CD9-FM7A1) at 1:50 dilution (2 μL of the antibody stock solution corresponds to labeling of 1e6 cells in a final volume of 100 µL), compared with isotype control antibody. FITC signal was used to evaluate the binding activity (QC tested).

    Protocol
  •  CD19 FACS

    Flow cytometric analysis of Human peripheral blood lymphocytes staining with PE anti-human CD3 antibody and FITC-Labeled Monoclonal Anti-Human CD19 Antibody, Mouse IgG2a (FMC63) (Cat. No. CD9-FM7A1) at 1:50 dilution (2 μL of the antibody stock solution corresponds to labeling of 1e6 cells in a final volume of 100 µL). FITC signal was used to evaluate the binding activity (QC tested).

    Protocol

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背景介绍

FMC63 is an IgG2a mouse monoclonal antibody specific for CD19, which is a target for the immunotherapy of B lineage leukaemias and lymphomas. FMC63 scFv is the most commonly used ectodomain component of CD19-specific CARs. So far, most of reported CART19 trials contain the anti-CD19 scFv derived from FMC63, including the two FDA-approved CARs Kymriah and Yescarta.

前沿进展

 
药物研发进展
  • 英文全称:

    B-lymphocyte antigen CD19

  • 中文全称:

    B淋巴细胞抗原CD19

  • 种类:

  • 上市药物数量:

    12 详情

  • 临床药物数量:

    310 详情

  • 最高研发阶段:

    申请上市

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