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Human IgG1 Kappa Isotype Control (mAb, carrier free, MALS verified)

COAMS
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  • 抗体来源（Source）

    Human IgG1 Kappa Isotype Control (mAb) is a chimeric monoclonal antibody recombinantly expressed from HEK293, which combines the variable region of a mouse monoclonal antibody with Human constant domain.

  • 亚型（Isotype）

    Human IgG1 | Human Kappa

  • 偶联（Conjugate）

    Unconjugated

  • 抗体类型（Antibody Type）

    Recombinant Monoclonal

  • 种属反应性（Reactivity）

    Human

  • 特异性（Specificity）

    This product is a specific antibody specifically reacts with DNP.

  • 应用（Application）

    Application
    Recommended Usage
    ELISA
    1-39 ng/mL

  • 纯度（Purity）

    95% as determined by SDS-PAGE.

    95% as determined by SEC-MALS.

  • 无菌（Sterility）

    The sterility testing was performed by membrane filtration method.

  • 纯化（Purification）

    Protein A purified / Protein G purified

  • 制剂（Formulation）

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

    25 mg or larger size will be supplied as liquid and shipped by dry ice. Please inquire the dry ice shipping cost.

  • 重构方法（Reconstitution）

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • 存储（Storage）

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.

  • 质量管理控制体系（QMS）

    1. 质量管理体系（ISO, GMP）
    2. 质量优势
    3. 质控流程

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  • 电泳（SDS-PAGE）

    IgG control SDS-PAGE

    Human IgG1 Kappa Isotype Control (mAb) on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).

  • SEC-MALS

    IgG control SEC-MALS

    The purity of Human IgG1 Kappa Isotype Control (mAb) (Cat. No. DNP-M2) is more than 95% and the molecular weight of this protein is around 135-160 kDa verified by SEC-MALS.

    Report

  • 活性（Bioactivity）-ELISA

    IgG control ELISA

    Immobilized DNP-Albumin Conjugate at 10 μg/mL (100 μL/well) can bind Human IgG1 Kappa Isotype Control (mAb) (Cat. No. DNP-M2) with a linear range of 1-10 ng/mL (QC tested).

    Protocol

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背景介绍

A hapten is a small molecule that can elicit an immune response only when conjugated with a large carrier such as a protein. Typical haptens include drugs, urushiol, quinone, steroids, etc. Peptides and non-protein antigens usually need conjugating to a carrier protein (such as BSA (bovine serum albumin) or KLH (keyhole limpet hemocyanin) to become good immunogens). Additionally, haptens should be administered with an adjuvant to ensure a high quality immune response. It is important that the hapten design (preserving greatly the chemical structure and spatial conformation of target compound), selection of the appropriate carrier protein and the conjugation method are key conditions for the desired specificity anti-hapten antibodies. We design anti-hapten antibodies based on the HaptenDB information.

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