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抗体来源（Source）
Monoclonal Anti-Payload PBD Antibody, Rabbit IgG (M1D08) is a Rabbit monoclonal antibody recombinantly expressed from HEK293 cells.
克隆号（Clone）
M1D08
种属（Species）
Rabbit
亚型（Isotype）
Rabbit IgG | Rabbit Kappa
偶联（Conjugate）
Unconjugated
抗体类型（Antibody Type）
Recombinant Monoclonal
免疫原（Immunogen）
PBD-BSA.
特异性（Specificity）
Specifically recognizes PBD.
应用（Application）
ApplicationRecommended UsageELISA0.06-10000 ng/mL纯度（Purity）
90% as determined by SDS-PAGE.
90% as determined by SEC-MALS.
纯化（Purification）
Protein A purified / Protein G purified
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
交叉验证（Cross Verification）
This product No cross-reactivity in ELISA with
IgG1-MMAF.
Doxorubicin-ADC.
Disitamab Vedotin (RC48).
Trastuzumab Deruxtecan.
Sacituzumab Govitecam.
Trastuzumab-DM1.质量管理控制体系（QMS）
数据展示
电泳（SDS-PAGE）
Monoclonal Anti-Payload PBD Antibody, Rabbit IgG (M1D08) on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Monoclonal Anti-Payload PBD Antibody, Rabbit IgG (M1D08) (Cat. No. PAD-MY2221) is more than 90% and the molecular weight of this protein is around 135-165 kDa verified by SEC-MALS.
Report
活性（Bioactivity）-ELISA
Immobilized Monoclonal Anti-Payload PBD Antibody, Rabbit IgG (M1D08) (Cat. No. PAD-MY2221) at 1 μg/mL, add Loncastuximab Tesirine in the 100% Human Serum and then add Biotinylated Human CD19 (20-291), His,Avitag, premium grade (Cat. No. CD9-H82E9) at 0.5 μg/mL. Detection was performed using HRP-conjugated Streptavidin (Acro, Cat. No. STN-NH913) (QC tested).
Protocol
Serial dilutions of (PBD)SG3199 were added into Monoclonal Anti-Payload PBD Antibody, Rabbit IgG (M1D08) (Cat. No. PAD-MY2221): Loncastuximab Tesirine binding reactions. The half maximal inhibitory concentration (IC50) is 0.7587 μg/mL (Routinely tested).
Protocol
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