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抗体来源（Source）
Anti-Trastuzumab Antibody, Mouse IgG1 (6F2F7-2) is a Mouse monoclonal antibody recombinantly expressed from HEK293 cells.
克隆号（Clone）
6F2F7-2
种属（Species）
Mouse
亚型（Isotype）
Mouse IgG1 | Mouse Kappa
抗体类型（Antibody Type）
Recombinant Monoclonal
免疫原（Immunogen）
Trastuzumab.
特异性（Specificity）
Recognizes Trastuzumab specifically.
应用（Application）
ApplicationRecommended UsageELISA0.2-25 ng/mL纯度（Purity）
95% as determined by SDS-PAGE.
纯化（Purification）
Protein A purified / Protein G purified
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 12 months under sterile conditions after reconstitution.
质量管理控制体系（QMS）
产品展示
电泳（SDS-PAGE）
Anti-Trastuzumab Antibody, Mouse IgG1 (6F2F7-2) on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
活性（Bioactivity）-ELISA
Immobilized Trastuzumab at 1 μg/mL (100 μL/well) can bind Anti-Trastuzumab Antibody, Mouse IgG1 (6F2F7-2) (Cat. No. TRB-Y5b) with a linear range of 0.19-6.25 ng/mL (QC tested).
Protocol
Serial dilutions of Anti-Trastuzumab Antibody, Mouse IgG1 (6F2F7-2) ( Cat. No. TRB-Y5b) was detected by Biotinylated Human Her2, His,Avitag (Cat. No. HE2-H82E2) with a half maximal inhibitory concentration (IC50) of 7.02 μg/mL (Routinely tested).
Protocol
活性（Bioactivity）-SPR
Trastuzumab captured on CM5 chip via Anti-human IgG Fc antibodies surface can bind Anti-Trastuzumab Antibody, Mouse IgG1 (6F2F7-2) (Cat. No. TRB-Y5b) with an affinity constant of 0.915 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
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