产品详情
- ItemsSize (2mg)Size(5mg X 2)Particle size2 μm2 μmPhysical appearancePowder mixturePowder mixtureAmount of Coupled Protein≈972 pmol (73.1 μg) Anti-RBD antibody/mg beads≈972 pmol (73.1 μg) Anti-RBD antibody/mg beadsBinding Capacity>192 pmol (10 μg) Spike RBD/mg beads>192 pmol (10 μg) Spike RBD/mg beadsFormulationPBS, pH7.4, with 10% TrehalosePBS, pH7.4, with 10% TrehaloseReconstitution2 mL sterile deionized water (1 mg beads/mL)5 mL sterile deionized water (1 mg beads/mL)
背景(Background)
The Human Anti-SARS-CoV-2 Spike RBD Antibody-coupled Magnetic Beads is produced by coupling biotinylated anti-RBD antibody to streptavidin-conjugated magnetic beads. The pre-coupled beads are ready to use for capturing spike protein from your sample with high specificity. The uniform size and large surface-to-volume ratio of the beads ensure highly efficent immunocapture in a simple, fast and convenient workflow with high reproducibilty of data.
应用说明(Application)
This product is intended for immunocapture, biopanning. This is a non-sterile product.
* The Isotype control (Cat. No. SMB-B01) is sold separately.
重构方法(Reconstitution)
See Certificate of Analysis (CoA) for detailed instruction.
存储(Storage)
Upon receipt, please store the Beads at -20°C. The shelf life is 1 year at -20 °C.
Please avoid more than 3 freeze-thaw cycles once reconstitution, immediate use after reconstitution is highly recommended.
原理(Assay Principles)
Application Method:
a) Reconstitute the Beads following the COA. Wash and re-suspend the beads to a certain concentration by adding your dilution buffer.
b) Add the prepared beads to your samples.
c) Beads can be separated from your samples afterwards using a magnetic plate.The magnetic beads technology makes use of the easy and efficient collection of beads in magnetic field to facilitate antibody purification in a simple workflow of “bind-wash-elute”. In contrast to common separation techniques, this method does not require columns or centrifugation, and is therefore ideal in high-throughput applications.
制剂(Formulation)
Please contact us for detailed information.
Contact us for customized product form or formulation.
质量管理控制体系(QMS)
产品展示
典型数据-Typical Data
Immobilized 73.1 μg Anti-RBD antibody/1mg beads can bind SARS-CoV-2 Spike RBD (Cat. No. SPD-C5259) with an EC50 of 0.4705 μg/mL.
Protocol
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