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- Genetically modified cell lines best reflect MOA (Mechanism of Action)
- Higher activity and larger assay window for robust and reproducible cell-based bioassay
- Comprehensive application data to support assay development and validation
- Full tracible record, stringent quality control and validated cell passage stability
- Parental cell line legally obtained from internationally recognized cell resource bank and commercially licensed
- Global commercial license assistance whenever regulatory filing is required
描述（Description）
The Human TSLP R (Luc) HEK293 Reporter Cell was engineered to not only express STAT5 signaling response element, but also express the receptors full length human TSLP R (Uniprot: Q9HC73-1) and IL-7 R alpha (Uniprot: P16871-1). When stimulated with human TSLP protein, the TSLP/TSLP R interaction drives STAT5-mediated luminescence. Inhibition of TSLP binding to TSLP R by either anti-TSLP or anti-TSLP R antibodies results in a decrease in luminescence.
应用说明（Application）
Screen for anti-human TSLP or anti-human TSLP R neutralizing antibody
生长特性（Growth Properties）
Adherent
筛选标记（Selection Marker）
Zeocin (100 μg/mL) + Puromycin (2 μg/mL)
培养基（Complete Growth Medium）
DMEM medium + 10% FBS
冻存液（Freeze Medium）
Serum-free cell cryopreservation medium
装量（Quantity）
1 vial contains at least 5×10^6 cells in 1 mL serum-free cryopreservation medium
存储（Storage）
Frozen in liquid nitrogen.
支原体检测（Mycoplasma Testing）
Negative
无菌检测（Sterility Testing）
Negative
使用说明（Instructions for Use）
See data sheet for detailed culturing and assay protocol.
产品展示
Receptor Assay
Co-expression analysis of human TSLP R and IL-7 R alpha on Human TSLP R (Luc) HEK293 Reporter Cell by FACS.
Cell surface staining was performed on Human TSLP R (Luc) HEK293 Reporter Cell or negative control cell using PE-labeled anti-TSLP R antibody and FITC-labeled anti-IL-7 R alpha antibody.Protocol
Application
Inhibition of human TSLP protein-induced reporter activity by anti-human TSLP neutralizing antibody.
This reporter cell was incubated with serial dilutions of antibodies in the presence of human TSLP protein (Cat. No. TSP-H52Hb) with a final concentration of 0.3 μg/mL. The EC50 of anti-human TSLP neutralizing antibody is approximately 0.55 μg/mL.Protocol
Signaling Bioassay
Response to human TSLP protein (RLU).
The Human TSLP R (Luc) HEK293 Reporter Cell was stimulated with serial dilutions of human TSLP protein (Cat. No. TSP-H52Hb). The EC50 was approximately 0.1392 μg/mL.Protocol
Response to human TSLP protein (Fold).
The Human TSLP R (Luc) HEK293 Reporter Cell was stimulated with serial dilutions of human TSLP protein (Cat. No. TSP-H52Hb). The max induction fold was approximately 45.15Protocol
Passage Stability
Passage stability analysis by Signaling Bioassay.
The continuously growing Human TSLP R (Luc) HEK293 Reporter Cell was stimulated with serial dilutions of human TSLP protein. Human TSLP protein stimulated response demonstrates passage stabilization (fold induction and EC50) across passage 10-26.Protocol
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