Anti-AAV2 Antibody ELISA Kit

产品详情

ID	Components	Size
PAV002-C01	Pre-coated AAV2 Capsid Protein Microplate	1 plate
PAV002-C02	Anti-AAV2 Antibody Standard	2.5 μg
PAV002-C03	Biotin-AAV2 Capsid Protein	10 μg
PAV002-C04	Streptavidin-HRP	50 μL
PAV002-C05	10×Washing Buffer	50 mL
PAV002-C06	2×Dilution Buffer	50 mL
PAV002-C07	Substrate Solution	12 mL
PAV002-C08	Stop Solution	7 mL

产品概述（Product Overview）
Anti-AAV2 Antibody ELISA Kit is based on the sandwich-ELISA method and is used to detect and quantitatively determine the Anti-AAV2 Antibody in serum. The kit is designed to provide reliable solutions for the immunogenicity studies and enrollment screening of AAV gene therapy products.
重构方法（Reconstitution）
Please see Certificate of Analysis for details of reconstitution instruction and specific concentration.
存储（Storage）
1. Unopened kit should be stored at 2℃-8℃ upon receiving.
2. Find the expiration date on the outside packaging and do not use reagents past their expiration date.
3. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
质量管理控制体系（QMS）

典型数据-Typical Data
Please refer to DS document for the assay protocol.
Detection of anti-AAV2 antibodies by sandwich-ELISA Assay.
Immobilized AAV2 Capsid Protein can bind Anti-AAV2 Antibody in 1:100 human serum, and then add Biotin-AAV2 Capsid Protein. Detection was performed using Streptavidin-HRP with sensitivity of 125 ng/mL.
The specific binding of PAV-A002 to Anti-AAV1/3/5/6/8/9 antibody was detected. The results showed that PAV-A002 only binds specifically to Anti-AAV2 antibody.

验证

批内差异（Intra-Assay Statistics）
Three samples of known concentration in blank human serum were tested six times on one plate to assess intra-assay precision, Intra-Assay Precision CV≤15%.
Three samples of known concentration in blank mouse serum were tested six times on one plate to assess intra-assay precision, Intra-Assay Precision CV≤15%.
Three samples of known concentration in blank monkey serum were tested six times on one plate to assess intra-assay precision, Intra-Assay Precision CV≤15%.
批间差异（Inter-Assay Statistics）
Three samples of known concentration in blank human serum were tested in six separate assays to assess inter-assay precision, Inter-Assay Precision CV≤15%.
Three samples of known concentration in blank mouse serum were tested in six separate assays to assess inter-assay precision, Inter-Assay Precision CV≤15%.
Three samples of known concentration in blank monkey serum were tested in six separate assays to assess inter-assay precision, Inter-Assay Precision CV≤15%.

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