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Mouse Anti-HSV-2 (strain 333) Antibody IgG Titer ELISA Assay Kit (gC)

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  • 组分(Materials Provided)

    ID
    Components
    Size
    RAS197-C01
    Pre-coated HSV-2 (strain 333) Envelope Glycoprotein C (gC) Microplate
    1 plate
    RAS197-C02
    HSV-2 (strain 333) (gC) Antibody Positive Control
    100 μL
    RAS197-C03
    HSV-2 (strain 333) (gC) Antibody Negative Control
    100 μL
    RAS197-C04
    HRP-Conjugated Antibody
    50 μL
    RAS197-C05
    10×Washing Buffer
    50 mL
    RAS197-C06
    Dilution Buffer
    50 mL
    RAS197-C07
    Substrate Solution
    12 mL
    RAS197-C08
    Stop Solution
    7 mL

  • 产品概述(Product Overview)

    Herpesvirus infections are widely spread throughout the world population. Herpes simplex virus (HSV) belongs to the α-herpesvirus subfamily. There are two main types of HSV, HSV-1 and HSV-2, which infect humans. HSV-2 mainly causes genital lesions, whereas HSV-1 is involved in both oral and genital infections. Glycoprotein C (gC) is a structural component of the herpes simplex virus type 2 (HSV-2) envelope that mediates binding of the virus to cell surface heparan sulfate or chondroitin sulfate. Also plays a role in host immune evasion by inhibiting the host complement cascade activation (By similarity).

  • 应用说明(Application)

    The kit is developed for titer measurement of Anti-HSV-2 Envelope Glycoprotein C (gC) antibody IgG in mouse serum.

    It is for research use only.

  • 存储(Storage)

    2-8℃

  • 原理(Assay Principles)

    This assay kit employs a standard indirect-ELISA format, providing a rapid detection of Anti-HSV-2 (strain 333) (gC) antibodies in Mouse serum by HSV-2 (strain 333) Envelope Glycoprotein C (gC). The Kit consists of Pre-coated HSV-2 (strain 333) Envelope Glycoprotein C (gC) Microplate,Positive Control,Negative Control and HRP-conjugated antibody.

    Your experiment will include 4 simple steps:

    a) The samples and Control are diluted by Dilution Buffer.Add your sample to the plate.

    b) Add the HRP-conjugated antibody diluted by Dilution Buffer to the plate.

    c) Wash the plate and add TMB or other colorimetric HRP substrate.

    d) Stop the substrate reaction by adding diluted acid. Absorbance (OD) is calculated as the absorbance at 450 nm minus the absorbance at 630 nm to remove background prior to statistical analysis. The OD Value reflects the amount of antibody bound.

  • 质量管理控制体系(QMS)

    1. 质量管理体系(ISO, GMP)
    2. 质量优势
    3. 质控流程

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