Mouse Anti-HSV-2 (strain 333) Antibody IgG Titer ELISA Assay Kit (gD)

组分（Materials Provided）

产品概述（Product Overview）

Herpesvirus infections are widely spread throughout the world population. Herpes simplex virus (HSV) belongs to the α-herpesvirus subfamily. There are two main types of HSV, HSV-1 and HSV-2, which infect humans. HSV-2 mainly causes genital lesions, whereas HSV-1 is involved in both oral and genital infections. Glycoprotein D (gD) is a structural component of the herpes simplex virus type 1 (HSV-1) envelope which is essential for virus entry and fusion with host cells. gD plays an important role by binding to the host receptors such as herpes virus entry mediator (HVEM) and nectin-1, a member of the immunoglobulin (Ig)-like cell adhesion molecules.

应用说明（Application）

The kit is developed for titer measurement of Anti-HSV-2 Envelope Glycoprotein D (gD) antibody IgG in mouse serum.

It is for research use only.

存储（Storage）

2-8℃

原理（Assay Principles）

This assay kit employs a standard indirect-ELISA format, providing a rapid detection of Anti-HSV-2 (strain 333) (gD) antibodies in Mouse serum by HSV-2 (strain 333) Envelope Glycoprotein D (gD). The Kit consists of Pre-coated HSV-2 (strain 333) Envelope Glycoprotein D (gD) Microplate,Positive Control,Negative Control and HRP-conjugated antibody.

Your experiment will include 4 simple steps:

a) The samples and Control are diluted by Dilution Buffer.Add your sample to the plate.

b) Add the HRP-conjugated antibody diluted by Dilution Buffer to the plate.

c) Wash the plate and add TMB or other colorimetric HRP substrate.

d) Stop the substrate reaction by adding diluted acid. Absorbance (OD) is calculated as the absorbance at 450 nm minus the absorbance at 630 nm to remove background prior to statistical analysis. The OD Value reflects the amount of antibody bound.

质量管理控制体系（QMS）

1. 质量管理体系（ISO, GMP）
2. 质量优势
3. 质控流程