Anti-CD19 (FMC63) CAR Immunogenicity ELISA Kit

组分（Materials Provided）

产品概述（Product Overview）

FMC63 is an IgG2a mouse monoclonal antibody specific for CD19, which is a target for the immunotherapy of B lineage leukaemias and lymphomas. FMC63 scFv is the most commonly used ectodomain component of CD19-specific CARs. So far, most of reported CART19 trials contain the anti-CD19 scFv derived from FMC63, including the two FDA-approved CARs Kymriah and Yescarta.Anti-fmc63 scFv antibody can specifically bind to the antigen recognition epitope of fmc63 scFv on anti-CD19 car, and it shows high specificity and sensitivity,it's used to detect the expression of fmc63 scFv derived car.

应用说明（Application）

This kit is developed for detection of Anti-CD19 (FMC63) antibodies in CAR Immunogenicity assay test. Detection and monitoring the level of ADA is important to fully understand the potential immune responses.

It is for research use only.

重构方法（Reconstitution）

Please see Certificate of Analysis for details of reconstitution instruction and specific concentration.

存储（Storage）

2-8℃

原理（Assay Principles）

This assay kit employs a bridging-ELISA format, providing a rapid detection of FMC63 ADA. The kit consists High-bind Plate and Mouse FMC63 scFv and FMC63 ADA Standard and Biotin-Mouse FMC63 scFv and Streptavidin-HRP and buffers.

Your experiment will include 4 simple steps:

a) Coat the plate with Mouse FMC63 scFv.

b) Add your molecule of interest to the tests.

c) Add Mouse FMC63 scFv-Biotin.

d) Add Streptavidin-HRP followed by TMB or other colorimetric HRP substrate.

质量管理控制体系（QMS）

1. 质量管理体系（ISO, GMP）
2. 质量优势
3. 质控流程