积分兑换
关注公众号
收藏产品 产品详情
组分（Materials Provided）
Box A(2-8°C)IDComponentsSizeRIPO-HWM002K-C01Basal medium A5.4mlRIPO-HWM002K-C02Basal medium B22.5mlRIPO-HWM002K-C03Basal medium C27mlRIPO-HWM002K-C04Basal medium M-M22.5mlBox B(-20°C)IDComponentsSizeRIPO-HWM002K-1-C01Medium F3mlRIPO-HWM002K-1-C02Supplement A0.6mlRIPO-HWM002K-1-C03Supplement B2.5mlRIPO-HWM002K-1-C04Supplement C3mlRIPO-HWM002K-1-C05Supplement M-M2.5ml产品描述（Product Details）
Human iPSC-Derived Cardiac Organoid differentiation Kit (Catalog No#: RIPO-HWM002K) allows hESC or iPSC to differentiate into cardiac organoids. Cardiac organoids are three-dimensional in vitro models with a cellular composition and structural organization that is representative of the human heart. This kit can produce 24 cardiac organoids in four steps. Differentiation was carried out by forming EBs from PSC at an ultra-low adsorption interface, and then changing the medium according to the instructions. A spontaneously beating cardiac organoid with a cavity can be seen between days 9 and 13 of the onset of differentiation. Cardiomyocyte, key cellular component of cardiac organoid, are responsible for the beating of the cardiac organoids. The beating rate of the formed cardiac organoids increases dramatically when stimulated with isoproterenol or forskolin, and there is a linear relationship between the concentration of the drug and the beating rate over a range of concentrations.
产品特征（Product Specification）
The basic medium of this differentiation kit is a serum-free, well-defined medium with minimal batch variation to which differentiation factors are added. This medium does not contain antibiotics, the addition of which may affect cardiac organoid differentiation.
存储（Storage）
The unopened Box A is stable for 12 months from the date of manufacture if stored under 2-8°C.
The unopened Box B is stable for 12 months from the date of manufacture if stored under -20°C.
The opened kit should be stored according to components table.- *请注意，为了支持完整的类器官培养和维持过程，本产品必须与Human iPSC-Derived Cardiac Organoid Maintenance Kit (Cat. No. RIPO-HWM004）一起使用。您可以点击此链接获取产品信息。
质量管理控制体系（QMS）
产品展示
产品示意（Product Diagram）
Protocol Diagram of cardiac organoid differentiation.
Organoid Histology and State
The cardiac organoids differentiated using the Human iPSC-Derived Cardiac Organoid Differentiation Kit (Ca. No. RIPO-HWM002K) show regular beating from day 7-13 of differentiation.
Marker Expression
The cardiac organoids differentiated using the Human iPSC-Derived Cardiac Organoid Differentiation Kit (Ca. No. RIPO-HWM002K) demonstrate the expression of cardiomyocyte marker cTnT (green) and vascular endothelial cell CD31(red)
Organoid Activity
The electrical activity of the cardiac organoids differentiated using the Human iPSC-Derived Cardiac Organoid Differentiation Kit (Ca. No. RIPO-HWM002K) was recorded using silicon probes (Ca. No. P64-7), revealing electrophysiological activity that closely resembles that of the actual human heart.
The cardiac organoids differentiated using the Human iPSC-Derived Cardiac Organoid Differentiation Kit (Ca. No. RIPO-HWM002K) show stable electrical activity
Organoid Application
E4031 caused the prolongation of QT interval on cardiac organoid measuring by MEA
Drug A cause damage to both cardiomyocytes and epithelial cells (Vascular) on Cardiac organoid.
用户评价 发表评论
联系我们 
项目合作