积分兑换
关注公众号
收藏产品 产品详情
产品参数（Product Specifications）
Assay TypeFRETAnalyteRNase AFormat96T/480TRegulatory StatusRUOSensitivity0.03125 pgStandard Curve Range0.03125pg-2pgAssay Time30 minSuitable Sample TypeFor the quantitative determination of RNase A in the environment, some biological materials, common molecular biological reagents such as reaction buffers, enzymes such as reverse transcriptase and RNA polymerase, and buffers for RNA purification and storageSample volume10 μL & 80 μL应用说明（Application）
RNase Activity Assay Kit (Fluorescence) is a convenient and sensitive assay tool to test the presence of RNase in buffers, reagents, and other components.
It is for research use only.
重构方法（Reconstitution）
Please see Certificate of Analysis for details of reconstitution instruction and specific concentration.
存储（Storage）
-20℃
组分（Materials Provided）
ItemsSize （96tests）Size（480tests）RNase Substrate2 nmol10 nmol10X Reaction Buffer for RNase10 mL10 mLRNase A (10μg/mL)100 μL500 μLTE Buffer (pH 7.0)1.5 mL6 mLNuclease-free Water10 mL50 mL原理（Assay Principles）
The RNase Activity Assay Kit (Fluorescence) is based on a fluorophore-labeled RNA substrate. When the sample does not contain RNase activity, the substrate is stable and does not produce a fluorescent signal; when the sample contains RNase activity, the substrate is degraded, resulting in a gradual enhanced fluorescence signal, the rate of increase in fluorescence signal is positively correlated with the number and activity of enzymes. Use a fluorescence microplate reader to measure at the wavelength of ex/em= 490/520 nm to determine whether the sample is contaminated by RNase.
质量管理控制体系（QMS）
产品展示
活性（Bioactivity）-Fluorescence
Please refer to DS document for the assay protocol.
Add 90 μL RNase Substrate Working Solution (mix RNase Substrate, 10×Reaction Buffer and Nuclease-free Water by 1:1:7 volume) to each 96-well plate, and add 10 μL RNase A standards (0-200 pg/mL×10 μL / well = 0-2 pg/well), incubate the plate in the fluorometer (BMG CLARIOstar) collecting real-time data at one minute intervals for 30 minutes at 37°C using the settings described in this section. The RNase Activity Assay can be evaluated in rigorous kinetic terms using real-time data.
This assay kit employs a standard detection of RNase A. Add 90 μL RNase Substrate Working Solution (mix RNase Substrate, 10×Reaction Buffer and Nuclease-free Water by 1:1:7 volume) to each 96-well plate, and add 10 μL of RNase A standards (0-200 pg/mL×10 μL/well = 0-2 pg/well), incubate for 30 minutes at 37°C. Then measure the fluorescence using the settings described in this section in a fluorometer (BMG CLARIOstar). Take RFU of standards as the ordinate and RNase concentration as the abscissa. Four parameters logistic are used to draw the standard curve. This following data is for reference only.(QC tested)
批内差异（Intra-Assay Statistics）
Eight replicates of each of seven samples containing different concentrations of RNase were tested in one assay, Intra-Assay Precision CV＜10%.
批间差异（Inter-Assay Statistics）
Eight replicates of each of seven samples containing different concentrations of RNase were tested in eight independent assays, Inter-Assay Precision CV<15%.
稳定性 (Stability)
The probe is freeze-thawed 3 times, the kit performance meets the requirements, the sensitivity is not reduced, and the CV< 10%.
回收率（Recovery）
Three different concentrations of RNase was spiked into four different kinds of matrixes. The range of the recovery rate is 80%~120%.
干扰效应(Interference effect)
DNase Activity Assay Kit (Fluorescence) (Cat. No. ASE-A002) and RNase Activity Assay Kit (Fluorescence) (Cat. No. ASE-A001) were used to detect nuclease residues in the same sample. No significant cross-reactivity or interference was observed.
- *Complete Your Research: DNase Activity Assay Kit (Fluorescence) (ACROBiosystems, cat#ASE-A002) is prepared.
用户评价 发表评论
联系我们 
项目合作