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产品参数（Product Specifications）
Assay TypeSandwich-ELISAAnalyteT7 RNA polymeraseFormat96T(8×12 strips)ReactivityHumanRegulatory StatusRUOSensitivity<0.78 ng/MlStandard Curve Range0.78 ng/mL-50 ng/mLAssay Time3 hr 20 minSuitable Sample TypeFor the quantitative determination of T7 RNA polymerase in Cell Culture Supernatants.Sample volume100 uL产品概述（Product Overview）
resDetect™ T7 RNA Polymerase ELISA Kit (Residue Testing) is based on the ELISA sandwich method and is designed to detect and quantitatively determine T7 RNA Polymerase residues in mRNA drugs. The kit contains T7 RNA Polymerase (ACROBiosystems, cat# T7E-E5143) to ensure accurate assay results and is designed to provide a reliable solution for monitoring and controlling T7 RNA Polymerase residues during the production of mRNA products. It can also be used as a universal detection tool for the quantitative determination of T7 RNA Polymerase.
应用说明（Application）
T7 RNA Polymerase ELISA Kit (Residue Testing)was developed for the detection and quantitative determination of T7 RNA Polymerase in mRNA preparation processing where T7 RNA Polymerase is used as an row agent.
It is for research use only.
存储（Storage）
The unopened kit is stable for 12 months from the date of manufacture if stored at 2°C to 8°C.
The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
组分（Materials Provided）
IDComponentsSizeRES018-C01Pre-coated Anti-T7 RNA polymerase Antibody Microplate1 plate(8×12 strips)RES018-C02T7 RNA polymerase Standard100 μLRES018-C03Biotin-Anti-T7 RNA polymerase Antibody150 μLRES018-C04Streptavidin-HRP50 μLRES018-C0520xWashing Buffer50 mLRES018-C06Biotin-Antibody and Streptavidin-HRP Dilution Buffer50 mLRES018-C07Standard and Sample Dilution Buffer (5x)30 mLRES018-C08Substrate Solution12 mLRES018-C09Stop Solution7 mL原理（Assay Principles）
This assay kit employs a standard sandwich-ELISA format, providing a rapid detection of T7 RNA polymerase. The kit consists of Pre-coated Anti-T7 RNA polymerase Antibody Microplate and T7 RNA polymerase Standard and Biotin-Anti-T7 RNA polymerase Antibody and Streptavidin-HRP and buffers.
Your experiment will include 6 simple steps:
a) Bring all reagents to room temperature(20℃-25℃) before use.
b) Add your sample to the plate and take the T7 RNA polymerase as standard. The samples and standard are diluted by Dilution Buffer.
c) Add the Biotin-Anti-T7 RNA polymerase Antibody diluted by Dilution Buffer to the plate.
d) Wash the plate and add the Streptavidin-HRP diluted by Dilution Buffer to the plate.
e) Wash the plate and add TMB.
f) Stop the substrate reaction by adding diluted acid. Absorbance (OD) is calculated by the absorbance at 450 nm minus the absorbance at 630 nm to remove background disturbance before statistical analysis. The OD Value reflects the amount of bound enzyme.
质量管理控制体系（QMS）
产品展示
典型数据-Typical Data
Please refer to DS document for the assay protocol.
For each experiment, a standard curve needs to be set for each micro-plate, and the specific OD value may vary depending on different laboratories, testers, or equipments. The following example data is for reference only.
批内差异（Intra-Assay Statistics）
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision, Intra-Assay Precision CV＜10%.
批间差异（Inter-Assay Statistics）
Three samples of known concentration were tested in three separate assays to assess inter-assay precision, Inter-Assay Precision CV<10%.
回收率（Recovery）
Three T7 RNA polymerases with different concentrations were tested to calculate the recovery rate.
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