选择语言
免费注册积分兑换
关注公众号
我的购物车
收藏产品

Human 4-1BB Ligand / TNFSF9 (71-254) Protein, His,Flag Tag, active trimer (MALS verified)

COAMS
DS
用户评价
货号-规格
价格
Qty.
合计0件 产品金额0
0
加入购物车
新品建议
增项建议

产品详情

  • 分子别名（Synonym）

    4-1BB Ligand,TNFSF9,CD137L

  • 表达区间及表达系统（Source）

    Human 4-1BB Ligand (71-254) Protein, His,Flag Tag (41L-H52D4) is expressed from human 293 cells (HEK293). It contains AA Arg 71 - Glu 254 (Accession # P41273-1).

    Predicted N-terminus: His

  • 蛋白结构（Molecular Characterization）

    This protein carries a polyhistidine tag at the N-terminus, followed by a flag tag.

    The protein has a calculated MW of 62.8 kDa. The protein migrates as 55-60 kDa under non-reducing (NR) condition (SDS-PAGE).

  • 偶联（Conjugate）

    Unconjugated

  • 内毒素（Endotoxin）

    Less than 1.0 EU per μg by the LAL method.

  • 纯度（Purity）

    95% as determined by SDS-PAGE.

  • 制剂（Formulation）

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • 重构方法（Reconstitution）

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • 存储（Storage）

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.

  • 质量管理控制体系（QMS）

    1. 质量管理体系（ISO, GMP）
    2. 质量优势
    3. 质控流程

产品展示

  • 电泳（SDS-PAGE）

    4-1BB Ligand SDS-PAGE

    Human 4-1BB Ligand (71-254) Protein, His,Flag Tag on SDS-PAGE under non-reducing (NR) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

  • SEC-MALS

    4-1BB Ligand SEC-MALS

    The purity of Human 4-1BB Ligand (71-254) Protein, His,Flag Tag (Cat. No. 41L-H52D4) is more than 85% and the molecular weight of this protein is around 55-70 kDa verified by SEC-MALS.

    Report

  • 活性（Bioactivity）-ELISA

    4-1BB Ligand ELISA

    Immobilized Human 4-1BB Ligand (71-254) Protein, His,Flag Tag (Cat. No. 41L-H52D4) at 2 μg/mL (100 μL/well) can bind Human 4-1BB, Fc Tag (Cat. No. 41B-H5258) with a linear range of 0.2-3 ng/mL (QC tested).

    Protocol

  • 活性（Bioactivity）-BLI

    4-1BB Ligand BLI

    Loaded Mouse 4-1BB, Fc Tag (Cat. No. 41B-M5258) on Protein A Biosensor, can bind Human 4-1BB Ligand (71-254) Protein, His,Flag Tag (Cat. No. 41L-H52D4) with an affinity constant of 18.6 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

    Protocol

  • 活性（Bioactivity）-CELL BASE

    4-1BB Ligand CELL

    Human 4-1BB Ligand (71-254) Protein, His,Flag Tag (Cat. No. 41L-H52D4) induce IL-8 secretion in HT1080 human CD137 cell line. The EC50 for this effect is 0.20-0.44 μg/mL (Routinely tested).

    Protocol

用户评价
发表评论

背景介绍

Tumor necrosis factor ligand superfamily member 9 (4-1BBL) is also known as 4-1BB ligand, CD137L or TNFSF9, which is a cytokine that binds to TNFRSF9. 4-1BBL is the high affinity ligand of 4-1BB. 4-1BBL induces the proliferation of activated peripheral blood T-cells. Also, 4-1BBL may have a role in activation-induced cell death (AICD). Furthermore, 4-1BBL may play a role in cognate interactions between T-cells and B-cells/macrophages. As for diseases, 4-1BBL is involved in cancers, infectious diseases and autoimmune diseases.

前沿进展

 
药物研发进展
  • 英文全称：

    Tumor necrosis factor ligand superfamily member 9

  • 中文全称：

    肿瘤坏死因子配体超家族成员9

  • 种类：

  • 上市药物数量：

    0 详情

  • 临床药物数量：

    2 详情

  • 最高研发阶段：

    临床二期

联系我们
项目合作
查看更多项目
  • 产品SKU
  • 产品详情
  • 产品展示
  • 用户评价
  • 背景介绍