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分子别名（Synonym）
Apolipoprotein E,APOE,Apo-E
表达区间及表达系统（Source）
Human APOE4, His Tag (APE-H52H9) is expressed from human 293 cells (HEK293). It contains AA Lys 19 - His 317 (Accession # P02649-1 (C130R)).
Predicted N-terminus: Lys 19
Request for sequence蛋白结构（Molecular Characterization）
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 36.2 kDa. The protein migrates as 35-42 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
偶联（Conjugate）
Unconjugated
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.
纯度（Purity）
90% as determined by SDS-PAGE.
90% as determined by SEC-MALS.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, 5 mM CHAPS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
质量管理控制体系（QMS）
产品展示
电泳（SDS-PAGE）
Human APOE4, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Human APOE4, His Tag (Cat. No. APE-H52H9) is more than 90% and the molecular weight of this protein is around 150-185 kDa verified by SEC-MALS.
Report
活性（Bioactivity）-BLI
Loaded Biotinylated Human TREM2, His,Avitag (Cat. No. TR2-H82E7) on SA Biosensor, can bind Human APOE4, His Tag (Cat. No. APE-H52H9) with an affinity constant of 90.2 nM as determined in BLI assay (ForteBio Octet Red96e) (QC tested).
Protocol
Loaded Human TREM2, Fc Tag (Cat. No. TR2-H5254) on Protein A Biosensor, can bind Human APOE4, His Tag (Cat. No. APE-H52H9) with an affinity constant of 85.5 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
Loaded Human LDL R, Fc Tag on Protein A Biosensor, can bind Human APOE4, His Tag (Cat. No. APE-H52H9) with an affinity constant of 42.8 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
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