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Biotinylated Human CA125 / MUC16 Protein, Fc,Avitag™

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  • 分子别名(Synonym)

    CA125,CA-125,CA125MUC-16,FLJ14303,MUC16,mucin 16,mucin-16

  • 表达区间及表达系统(Source)

    Biotinylated Human CA125, Fc,Avitag (CA5-H82F4) is expressed from human 293 cells (HEK293). It contains AA Gly 12660 - Met 12923 (Accession # Q8WXI7-1).

    Predicted N-terminus: Gly 12660

    Request for sequence
  • 蛋白结构(Molecular Characterization)

    CA125 Structure

    This protein carries a human IgG1 Fc tag at the C-terminus, followed by an Avi tag (Avitag™).

    The protein has a calculated MW of 56.9 kDa. The protein migrates as 75-100 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • 偶联(Conjugate)

    Biotin

  • 标记(Labeling)

    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

  • 内毒素(Endotoxin)

    Less than 0.1 EU per μg by the LAL method.

  • 纯度(Purity)

    >95% as determined by SDS-PAGE.

  • 制剂(Formulation)

    Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • 重构方法(Reconstitution)

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • 存储(Storage)

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • 质量管理控制体系(QMS)

    1. 质量管理体系(ISO, GMP)
    2. 质量优势
    3. 质控流程

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  • 电泳(SDS-PAGE)

    CA125 SDS-PAGE

    Biotinylated Human CA125, Fc,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

  • 活性(Bioactivity)-ELISA

     CA125 ELISA

    Immobilized Human Mesothelin, His Tag at 10 μg/mL (100 μL/well) can bind Biotinylated Human CA125, Fc,Avitag (Cat. No. CA5-H82F4) with a linear range of 2-78 ng/mL (QC tested).

    Protocol
  • 活性(Bioactivity)-SPR

     CA125 SPR

    Human Mesothelin (296-580), His Tag, premium grade (Cat. No. MSN-H522a) immobilized on CM5 Chip can bind Biotinylated Human CA125, Fc,Avitag (Cat. No. CA5-H82F4) with an affinity constant of 0.66 μM as determined in a SPR assay (Biacore 8K) (Routinely tested).

    Protocol

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背景介绍

The CA125, also known as the MUC16, is a mucin protein that may be found in type I transmembrane or secreted forms that are used monitor the progress of epithelial ovarian cancer therapy. The CA 125 molecule is almost certainly a glycoprotein with a predominance of O-linkages. It is heterogeneous with regard to both size and charge, most likely due to continuous deglycosylation of side chains during its life-span in bodily fluids. It exists as a very large complex (perhaps as much as 4 million daltons) under natural conditions.

前沿进展

 
药物研发进展
  • 英文全称:

    Mucin-16

  • 中文全称:

    粘蛋白-16

  • 种类:

  • 上市药物数量:

    0 详情

  • 临床药物数量:

    7 详情

  • 最高研发阶段:

    临床三期

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