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Human / Cynomolgus / Rhesus macaque CD28 Protein, His Tag, active dimer (MALS verified)

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  • 分子别名（Synonym）

    CD28,Tp44

  • 表达区间及表达系统（Source）

    Human / Cynomolgus / Rhesus macaque CD28 Protein, His Tag (CD8-H52H3) is expressed from human 293 cells (HEK293). It contains AA Asn 19 - Pro 152 (Accession # P10747-1). In the region Asn 19 - Pro 152, the AA sequence of Human, Cynomolgus and Rhesus macaque CD28 are homologus.

    Predicted N-terminus: Asn 19

    Request for sequence

  • 蛋白结构（Molecular Characterization）

    CD28 Structure

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 20.4 kDa. The protein migrates as 38-45 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • 偶联（Conjugate）

    Unconjugated

  • 内毒素（Endotoxin）

    Less than 1.0 EU per μg by the LAL method.

  • 纯度（Purity）

    90% as determined by SDS-PAGE.

    90% as determined by SEC-MALS.

  • 制剂（Formulation）

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • 重构方法（Reconstitution）

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • 存储（Storage）

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.

  • 质量管理控制体系（QMS）

    1. 质量管理体系（ISO, GMP）
    2. 质量优势
    3. 质控流程

产品展示

  • 电泳（SDS-PAGE）

    CD28 SDS-PAGE

    Human / Cynomolgus / Rhesus macaque CD28 Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).

  • SEC-MALS

    CD28 SEC-MALS

    The purity of Human / Cynomolgus / Rhesus macaque CD28 Protein, His Tag (Cat. No. CD8-H52H3) is more than 90% and the molecular weight of this protein is around 65-75 kDa verified by SEC-MALS.

    Report

  • 活性（Bioactivity）-ELISA

    CD28 ELISA

    Immobilized Human / Cynomolgus / Rhesus macaque CD28 Protein, His Tag (Cat. No. CD8-H52H3) at 2 μg/mL (100 μL/well) can bind Monoclonal Anti-Human CD28 Antibody, premium grade (Cat. No. CD8-M120b) with a linear range of 0.01-1 ng/mL (QC tested).

    Protocol

  • 活性（Bioactivity）-BLI

    CD28 BLI

    Loaded Human B7-1, Fc Tag (Cat. No. B71-H5259) on Protein A Biosensor, can bind Human / Cynomolgus / Rhesus macaque CD28 Protein, His Tag (Cat. No. CD8-H52H3) with an affinity constant of 4.5 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

    Protocol

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背景介绍

T-cell-specific surface glycoprotein CD28 is also known as TP44, is a single-pass type I membrane protein which contains one Ig-like V-type (immunoglobulin-like) domain. is one of the molecules expressed on T cells that provide co-stimulatory signals, which are required for T cell activation. CD28 is the receptor for CD80 (B7.1) and CD86 (B7.2). When activated by Toll-like receptor ligands, the CD80 expression is upregulated in antigen presenting cells (APCs). The CD86 expression on antigen presenting cells is constitutive. CD28 is the only B7 receptor constitutively expressed on naive T cells.

前沿进展

 
药物研发进展
  • 英文全称：

    T-cell-specific surface glycoprotein CD28

  • 中文全称：

    T细胞特异性表面糖蛋白CD28

  • 种类：

  • 上市药物数量：

    0 详情

  • 临床药物数量：

    25 详情

  • 最高研发阶段：

    临床二期

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