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分子别名（Synonym）
CD4,CD4mut,LEU3
表达区间及表达系统（Source）
FITC-Labeled Human CD4, Fc Tag (CD4-HF255) is expressed from human 293 cells (HEK293). It contains AA Lys 26 - Pro 396 (Accession # P01730). It is the FITC labeled form of Human CD4, Fc Tag (CD4-H5259).
Predicted N-terminus: Lys 26
Request for sequence蛋白结构（Molecular Characterization）
This protein carries a human IgG1 Fc tag at the C-terminus.
The protein has a calculated MW of 67.7 kDa. The protein migrates as 67-80 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
偶联（Conjugate）
FITC
Excitation source: 488 nm spectral line, argon-ion laser
Excitation Wavelength: 488 nm
Emission Wavelength: 535 nm
标记（Labeling）
The primary amines in the side chains of lysine residues and the N-terminus of the protein are conjugated with FITC using standard chemical labeling method. The residual FITC is removed by molecular sieve treatment during purification process.
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.
纯度（Purity）
95% as determined by SDS-PAGE.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please protect from light and avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
质量管理控制体系（QMS）
产品展示
电泳（SDS-PAGE）
FITC-Labeled Human CD4, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
活性（Bioactivity）-ELISA
Immobilized Ibalizumab (Human IgG4) at 1 μg/mL (100 μL/well) can bind FITC-Labeled Human CD4, Fc Tag (Cat. No. CD4-HF255) with a linear range of 20-313 ng/mL (QC tested).
Protocol
Immobilized HIV-1 [HIV-1/Clade C (16055)] GP120, His Tag (Cat. No. GP5-V15224) at 10 μg/mL (100 μL/well) can bind FITC-Labeled Human CD4, Fc Tag (Cat. No. CD4-HF255) with a linear range of 0.039-0.313 μg/mL (Routinely tested).
Protocol
活性（Bioactivity）-FACS
2e5 of CD4-CAR-293 cells transfected with anti-CD4-scFv were stained with 100 μL of 1 μg/mL of FITC-Labeled Human CD4 Protein, Fc Tag (Cat. No. CD4-HF255) and negative control protein respectively. FITC signal was used to evaluate the binding activity (QC tested).
Protocol
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