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Human Complement C5 Protein, His Tag (MALS verified)

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  • 分子别名（Synonym）

    Complement C5,C5,CPAMD4

  • 表达区间及表达系统（Source）

    Human Complement C5, His Tag (CO5-H52Ha) is expressed from human 293 cells (HEK293). It contains AA Gln 19 - Cys 1676 (Accession # P01031-1).

    Predicted N-terminus: Gln 19 (β chain) & Thr 678 (α chain)

    Request for sequence

  • 蛋白结构（Molecular Characterization）

    Complement C5 Structure

    This protein carries a polyhistidine tag at the C-terminus.

    The mature form of Complement C5 is a disulfide-linked heterodimer composed of proteolytically cleaved α and β chain. Each α and β chain has a calculated MW of 73.9 kDa (β chain) and 114.4 kDa (α chain). The protein migrates as 66 kDa, 110 kDa and >180kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition, and >180 kDa under non-reducing (NR) condition (SDS-PAGE).

  • 偶联（Conjugate）

    Unconjugated

  • 内毒素（Endotoxin）

    Less than 1.0 EU per μg by the LAL method.

  • 纯度（Purity）

    90% as determined by SDS-PAGE.

  • 制剂（Formulation）

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • 重构方法（Reconstitution）

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • 存储（Storage）

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.

  • 质量管理控制体系（QMS）

    1. 质量管理体系（ISO, GMP）
    2. 质量优势
    3. 质控流程

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  • 电泳（SDS-PAGE）

    Complement C5 SDS-PAGE

    Human Complement C5, His Tag on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%undefined.

  • SEC-MALS

    Complement C5 SEC-MALS

    The purity of Human Complement C5, His Tag (Cat. No. CO5-H52Ha) is more than 85% and the molecular weight of this protein is around 180-220 kDa verified by SEC-MALS.

    Report

  • 活性（Bioactivity）-ELISA

    Complement C5 ELISA

    Immobilized Human Complement C5, His Tag (Cat. No. CO5-H52Ha) at 2 μg/mL (100 μL/well) can bind Anti-C5a (Human IgG1) with a linear range of 0.2-3 ng/mL (QC tested).

    Protocol

  • Complement C5 ELISA

    Immobilized Human Complement C5, His Tag (Cat. No. CO5-H52Ha) at 2 μg/mL (100 μL/well) can bind Eculizumab Biosimilar with a linear range of 0.1-4 ng/mL (Routinely tested).

    Protocol

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背景介绍

Derived from proteolytic degradation of complement C5, C5 anaphylatoxin is a mediator of local inflammatory process. C5 precursor is first processed by the removal of 4 basic residues, forming two chains, beta and alpha, linked by a disulfide bond. C5 convertase activates C5 by cleaving the alpha chain, releasing C5a anaphylatoxin and generating C5b (beta chain + alpha' chain). Activation of C5 by a C5 convertase initiates the spontaneous assembly of the late complement components, C5-C9, into the membrane attack complex. C5b has a transient binding site for C6. The C5b-C6 complex is the foundation upon which the lytic complex is assembled. The C5a anaphylatoxin interacts with C5AR1 and tick complement inhibitor. C5a is also a potent chemokine which stimulates the locomotion of polymorphonuclear leukocytes and directs their migration toward sites of inflammation.

前沿进展

 
药物研发进展
  • 英文全称：

    Complement C5

  • 中文全称：

    补体C5

  • 种类：

  • 上市药物数量：

    8 详情

  • 临床药物数量：

    18 详情

  • 最高研发阶段：

    批准上市

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