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分子别名（Synonym）
CTLA4,CD152
表达区间及表达系统（Source）
Biotinylated Cynomolgus / Rhesus macaque CTLA-4, Avitag,His Tag (CT4-C82E5) is expressed from human 293 cells (HEK293). It contains AA Ala 37 - Ser 160 (Accession # G7PL88-1). In the region Ala 37 - Ser 160, the AA sequence of Cynomolgus and Rhesus macaque CTLA-4 are homologus.
Predicted N-terminus: Ala 37
Request for sequence蛋白结构（Molecular Characterization）
This protein carries an Avi tag (Avitag™) at the C-terminus, followed by a polyhistidine tag.
The protein has a calculated MW of 15.9 kDa. The protein migrates as 20-26 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
偶联（Conjugate）
Biotin
标记（Labeling）
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.
纯度（Purity）
>95% as determined by SDS-PAGE.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
质量管理控制体系（QMS）
产品展示
电泳（SDS-PAGE）
Biotinylated Cynomolgus / Rhesus macaque CTLA-4, Avitag,His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
活性（Bioactivity）-ELISA
Immobilized Human B7-1, Fc Tag (Cat. No. B71-H5259) at 2 μg/mL (100 μL/well) can bind Biotinylated Cynomolgus / Rhesus macaque CTLA-4, Avitag,His Tag (Cat. No. CT4-C82E5) with a linear range of 2-31 ng/mL (QC tested).
Protocol
Immobilized Human B7-2, Fc Tag (Cat. No. CD6-H5257) at 5 μg/mL (100 μL/well) can bind Biotinylated Cynomolgus / Rhesus macaque CTLA-4, Avitag,His Tag (Cat. No. CT4-C82E5) with a linear range of 2-63 ng/mL (Routinely tested).
Protocol
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背景介绍
前沿进展
药物研发进展 - 英文全称：
Cytotoxic T-Lymphocyte-Associated Antigen 4
- 中文全称：
细胞毒性T淋巴细胞相关抗原4
- 种类：
- 上市药物数量：
4 详情
- 临床药物数量：
64 详情
- 最高研发阶段：
临床二期
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