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分子别名（Synonym）
Fc gamma RIIIB , CD16b (NA1),FCGR3B,CD16B,FCG3B,FCGR3,FCG3,IGFR3
表达区间及表达系统（Source）
Human CD16b (NA1), His Tag (CDB-H5227) is expressed from human HEK293 cells. It contains AA Gly 17 - Ser 200 (Accession # AAA35881.1). The NA1 form of the CD16b differ with the NA2 form of CD16b in AA36, 65, 82, and 106. The NA1 form carries R36, N65, D82, and V106, while the NA2 form carries S36, S65, N82, and I106.
Predicted N-terminus: Gly 17
Request for sequence蛋白结构（Molecular Characterization）
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 22.8 kDa. The protein migrates as 35-45 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
偶联（Conjugate）
Unconjugated
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.
纯度（Purity）
95% as determined by SDS-PAGE.
90% as determined by SEC-MALS.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 12 months under sterile conditions after reconstitution.
质量管理控制体系（QMS）
产品展示
电泳（SDS-PAGE）
Human CD16b (NA1), His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Human CD16b (NA1), His Tag (Cat. No. CDB-H5227) is more than 90% and the molecular weight of this protein is around 30-45 kDa verified by SEC-MALS.
Report
活性（Bioactivity）-SPR
Immobilized Human CD16b (NA1), His Tag (Cat. No. CDB-H5227) on CM5 Chip via anti-His antibody, can bind Rituximab with an affinity constant of 8.17 μM as determined in a SPR assay (Biacore T200) (QC tested).
Protocol
Rituximab captured on Protein A Chip can bind Human CD16b (NA1), His Tag (Cat. No. CDB-H5227) with an affinity constant of 8.97 μM as determined in SPR assay (Biacore 8K) (Routinely tested).
Protocol
活性（Bioactivity）-BLI
Loaded Human CD16b (NA1), His Tag (Cat. No. CDB-H5227) on HIS1K Biosensor, can bind Rituximab with an affinity constant of 18 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
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背景介绍
CD16B is also kown as FCGR3B and FCG3B, is expressed specifically by polymorphonuclear leukocytes (neutrophils) and stimulated eosinophils. CD16B is the low affinity receptor for the Fc region of immunoglobulins gamma. FCGR3B binds complexed or aggregated IgG and also monomeric IgG. Contrary to III-A, FCG3B is not capable to mediate antibody-dependent cytotoxicity and phagocytosis. CD16B may serve as a trap for immune complexes in the peripheral circulation which does not activate neutrophils.
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