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分子别名（Synonym）
FcRn,FCGRT & B2M
表达区间及表达系统（Source）
Cynomolgus / Rhesus macaque FCGRT&B2M Heterodimer Protein, His Tag, is expressed from human 293 cells (HEK293). It contains AA Ala 24 - Ser 297 (FCGRT) & Ile 21 - Met 119 (B2M) (Accession # Q8SPV9-1 (FCGRT) & Q8SPW0-1 (B2M)). In the region Ala 24 - Ser 297 (FCGRT) & Ile 21 - Met 119 (B2M), the AA sequence of Cynomolgus and Rhesus macaque FCGRT&B2M are homologus.
Predicted N-terminus: Ala 24 (FCGRT) & Ile 21 (B2M)
Request for sequence蛋白结构（Molecular Characterization）
Cynomolgus / Rhesus macaque FCGRT&B2M Heterodimer Protein, His Tag, produced by co-expression of FCGRT and B2M, has a calculated MW of 34.1 kDa (FCGRT) and 11.6 kDa (B2M). Subunit FCGRT is fused with a polyhistidine tag at the C-terminus and subunit Beta-2 microglobulin (B2M) contains no tag at the C-terminus. The reducing (R) protein migrates as 34 kDa (FCGRT) and 12 kDa (B2M) respectively due to glycosylation.
偶联（Conjugate）
Unconjugated
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.
纯度（Purity）
95% as determined by SDS-PAGE.
90% as determined by SEC-MALS.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 12 months under sterile conditions after reconstitution.
质量管理控制体系（QMS）
产品展示
电泳（SDS-PAGE）
Cynomolgus / Rhesus macaque FCGRT&B2M Heterodimer Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%undefined.
SEC-MALS
The purity of Cynomolgus / Rhesus macaque FCGRT&B2M Heterodimer Protein, His Tag (Cat. No. FCM-C52W9) is more than 90% and the molecular weight of this protein is around 38-56 kDa verified by SEC-MALS.
Report
活性（Bioactivity）-SPR
Captured Cynomolgus / Rhesus macaque FCGRT&B2M Heterodimer Protein, His Tag (Cat. No. FCM-C52W9) on CM5 Chip via Anti-His antibody can bind Herceptin® with an affinity constant of 1.14 μM as determined in SPR assay (Biacore T200) (Routinely tested).
Protocol
活性（Bioactivity）-BLI
Loaded Cynomolgus / Rhesus macaque FCGRT&B2M Heterodimer Protein, His Tag (Cat. No. FCM-C52W9) on SA Biosensor via Biotin his antibody, can bind Herceptin with an affinity constant of 0.16 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
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