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分子别名（Synonym）
FcRn,FCGRT & B2M
表达区间及表达系统（Source）
Porcine FCGRT&B2M Heterodimer Protein, His Tag&Strep II Tag (FCM-P5280) is expressed from human 293 cells (HEK293). It contains AA Asp 16 - Ser 289 (FCGRT) & Val 21 - His 118 (B2M) (Accession # Q6XAV9-1 (FCGRT) & Q07717-1 (B2M)).
Predicted N-terminus: Asp 16 (FCGRT) & Val 21 (B2M)
Request for sequence蛋白结构（Molecular Characterization）
Porcine FCGRT&B2M Heterodimer Protein, His Tag&Strep II Tag, produced by co-expression of FCGRT and B2M, has a calculated MW of 32.3 kDa (FCGRT) and 12.8 kDa (B2M). Subunit FCGRT is fused with a polyhistidine tag at the C-terminus and subunit Beta-2 microglobulin (B2M) is fused with a Strep II tag at the C-terminus. The reducing (R) protein migrates as 32-35 kDa (FCGRT) and 14 kDa (B2M) respectively.
偶联（Conjugate）
Unconjugated
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.
纯度（Purity）
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
质量管理控制体系（QMS）
数据展示
电泳（SDS-PAGE）
Porcine FCGRT&B2M Heterodimer Protein, His Tag&Strep II Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%undefined.
SEC-MALS
The purity of Porcine FCGRT&B2M Heterodimer Protein, His Tag&Strep II Tag (Cat. No. FCM-P5280) is more than 90% and the molecular weight of this protein is around 40-50 kDa verified by SEC-MALS.
Report
活性（Bioactivity）-SPR
Porcine FCGRT&B2M Heterodimer Protein, His Tag&Strep II Tag (Cat. No. FCM-P5280) immobilized on CM5 Chip can bind Trastuzumab with an affinity constant of 95.1 nM as determined in a SPR assay (Biacore 8K) (QC tested).
Protocol
Trastuzumab immobilized on CM5 Chip can bind Porcine FCGRT&B2M Heterodimer Protein, His Tag&Strep II Tag (Cat. No. FCM-P5280) with an affinity constant of 81.5 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
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