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Human cytomegalovirus (strain AD169) (HHV-5) Glycoprotein H&L&O, His Tag (MALS verified)

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  • 分子别名(Synonym)

    Glycoprotein H & Glycoprotein L & Glycoprotein O/gH & gL & gO (HCMV)

  • 表达区间及表达系统(Source)

    Human cytomegalovirus (strain AD169) (HHV-5) Glycoprotein H&L&O, His Tag (GLO-V5243) is expressed from human 293 cells (HEK293). It contains AA Lys 31 - Gln 466 & Val 31 - Arg 278 & Arg 24 - Leu 720 (Accession # P12824 & P16832 & P16750).

    Predicted N-terminus: His

  • 蛋白结构(Molecular Characterization)

    This protein carries a polyhistidine tag at the N-terminus.

    The protein has a calculated MW of 162.2 kDa. The protein migrates as 180 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • 偶联(Conjugate)

    Unconjugated

  • 内毒素(Endotoxin)

    Less than 1.0 EU per μg by the LAL method.

  • 纯度(Purity)

    90% as determined by SDS-PAGE.

  • 制剂(Formulation)

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • 重构方法(Reconstitution)

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • 存储(Storage)

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • 质量管理控制体系(QMS)

    1. 质量管理体系(ISO, GMP)
    2. 质量优势
    3. 质控流程

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  • 电泳(SDS-PAGE)

    Glycoprotein H & Glycoprotein L & Glycoprotein O/gH & gL & gO (HCMV) SDS-PAGE

    Human cytomegalovirus (strain AD169) (HHV-5) Glycoprotein H&L&O, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).

  • SEC-MALS

    Glycoprotein H & Glycoprotein L & Glycoprotein O/gH & gL & gO (HCMV) SEC-MALS

    The purity of Human cytomegalovirus (strain AD169) (HHV-5) Glycoprotein H&L&O, His Tag (Cat. No. GLO-V5243) is more than 85% and the molecular weight of this protein is around 195-235 kDa verified by SEC-MALS.

    Report
  • 活性(Bioactivity)-ELISA

     Glycoprotein H & Glycoprotein L & Glycoprotein O/gH & gL & gO (HCMV) ELISA

    Immobilized Human cytomegalovirus (strain AD169) (HHV-5) Glycoprotein H&L&O, His Tag (Cat. No. GLO-V5243) at 1 μg/mL (100 μL/well) can bind Anti-HCMV gH [HCMV16], Human, IgG1 with a linear range of 0.05-2 ng/mL (QC tested).

    Protocol
  • 活性(Bioactivity)-SPR

     Glycoprotein H & Glycoprotein L & Glycoprotein O/gH & gL & gO (HCMV) SPR

    Human cytomegalovirus (strain AD169) (HHV-5) Glycoprotein H&L&O, His Tag (Cat. No. GLO-V5243) immobilized on CM5 Chip can bind Human PDGF R alpha, His Tag (Cat. No. PDA-H52H7) with an affinity constant of 2.52 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).

    Protocol

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背景介绍

Human cytomegalovirus (HCMV), a prototypical beta-herpes virus, is a major cause of morbidity and mortality in immunocompromised transplant recipients and congenitally infected fetuses.Different HCMV glycoprotein complexes are capable of meditating entry through interaction with cell-specific receptors on a panel of host cells. The pentamer (gH/gL/UL128/UL130/UL131A) mediates HCMV entry into epithelial, endothelial, and myeloid cells by its binding to neuropilin 2 (Nrp2). The trimer (gH/gL/gO) can mediate infection of all cell types by binding to platelet-derived growth factor-alpha (PDGFRα). Both pentamer and trimer need to interact with the glycoprotein B (gB), the fusogenic protein, to trigger the virus and host cell membrane fusion.

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