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Biotinylated Human Growth Hormone R (GHR) Protein, His Tag, ultra sensitivity, primary amine labeling

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  • 分子别名(Synonym)

    GHR,GHBP,GH receptor

  • 表达区间及表达系统(Source)

    MABSol® Biotinylated Human GHR Protein, His Tag, primary amine labeling (GHR-H8222) is expressed from human HEK293 cells. It contains AA Ala 27 - Tyr 264 (Accession # P10912-1). It is the biotinylated form of Human Growth Hormone R (GHR) Protein (GHR-H5222).

    Predicted N-terminus: Ala 27

    Request for sequence

  • 蛋白结构(Molecular Characterization)

    Growth Hormone R Structure

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 29.6 kDa. The protein migrates as 40-60 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • 偶联(Conjugate)

    Biotin

  • 标记(Labeling)

    The primary amines in the side chains of lysine residues and the N-terminus of the protein are conjugated with biotins using standard chemical labeling method. A standard biotin reagent (13.5 angstroms) is used in this product.

  • 内毒素(Endotoxin)

    Less than 1.0 EU per μg by the LAL method.

  • 纯度(Purity)

    90% as determined by SDS-PAGE.

  • 制剂(Formulation)

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • 重构方法(Reconstitution)

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • 存储(Storage)

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • 质量管理控制体系(QMS)

    1. 质量管理体系(ISO, GMP)
    2. 质量优势
    3. 质控流程

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  • 电泳(SDS-PAGE)

    Growth Hormone R SDS-PAGE

    Biotinylated Human GHR Protein, His Tag, primary amine labeling on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.

  • 活性(Bioactivity)-ELISA

     Growth Hormone R ELISA

    Immobilized Human GH, Tag Free at 2 μg/mL (100 μL/well) can bind Biotinylated Human GHR Protein, His Tag, primary amine labeling (Cat. No. GHR-H8222) with a linear range of 0.5-8 ng/mL (QC tested).

    Protocol

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背景介绍

Growth hormone receptor (GHR) is also known as somatotropin receptor, growth hormone-binding protein (GHBR), which belongs to the type I cytokine receptor family or Type 1 subfamily. GHR contains one fibronectin type-III domain. GHR / GHBR is expressed in various tissues with high expression in liver and skeletal muscle. The soluble form (GHBP) is produced by phorbol ester-promoted proteolytic cleavage at the cell surface (shedding) by ADAM17/TACE. GHR is receptor for pituitary gland growth hormone involved in regulating postnatal body growth. On ligand binding, couples to the JAK2/STAT5 pathway. The soluble form (GHBP) acts as a reservoir of growth hormone in plasma and may be a modulator/inhibitor of GH signaling.

前沿进展

 
药物研发进展
  • 英文全称:

    Growth hormone receptor

  • 中文全称:

    生长激素受体

  • 种类:

  • 上市药物数量:

    28 详情

  • 临床药物数量:

    32 详情

  • 最高研发阶段:

    批准上市

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