积分兑换
关注公众号
收藏产品 产品详情
分子别名（Synonym）
IgG1
表达区间及表达系统（Source）
Mouse IgG Fc, Tag Free (IG1-M5208) is expressed from human 293 cells (HEK293). It contains AA Val 111 - Lys 324 (Accession # AAK53870.1).
Predicted N-terminus: Val 111
Request for sequence蛋白结构（Molecular Characterization）
This protein carries no "tag".
The protein has a calculated MW of 24.3 kDa. The protein migrates as 28-30 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
偶联（Conjugate）
Unconjugated
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.
纯度（Purity）
>95% as determined by SDS-PAGE.
>95% as determined by SEC-MALS.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 25 mM Arginine, 150 mM NaCl, pH7.5 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
质量管理控制体系（QMS）
数据展示
电泳（SDS-PAGE）
Mouse IgG Fc, Tag Free on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
SEC-MALS
The purity of Mouse IgG Fc, Tag Free (Cat. No. IG1-M5208) is more than 95% and the molecular weight of this protein is around 45-65 kDa verified by SEC-MALS.
Report
活性（Bioactivity）-BLI
Loaded Mouse FCGRT&B2M Heterodimer Protein, His Tag (Cat. No. FCM-M52W8) on NTA Biosensor, can bind Mouse IgG Fc, Tag Free (Cat. No. IG1-M5208) with an affinity constant of 17.9 nM as determined in BLI assay (ForteBio Octet Red96e) (QC tested).
Protocol
用户评价 发表评论
联系我们 
项目合作