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Mouse IgG2a Fc Protein, Tag Free (MALS verified)

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IGA-M5207-200ug
¥2835.00
IGA-M5207-1mg
¥6615.00
合计0件 产品金额¥ 0

产品详情

  • 分子别名(Synonym)

    IgG2A

  • 表达区间及表达系统(Source)

    Mouse IgG2a Fc, Tag Free (IGA-M5207) is expressed from human 293 cells (HEK293). It contains AA Glu 98 - Lys 330 (Accession # CAC20702).

    Predicted N-terminus: Glu 98

    Request for sequence
  • 蛋白结构(Molecular Characterization)

    IgG2a Fc Structure

    This protein carries no "tag".

    The protein has a calculated MW of 26.4 kDa. The protein migrates as 30-35 kDa under reducing (R) condition, and 55-60 kDa under non-reducing (NR) condition (SDS-PAGE) due to glycosylation.

  • 偶联(Conjugate)

    Unconjugated

  • 内毒素(Endotoxin)

    Less than 1.0 EU per μg by the LAL method.

  • 纯度(Purity)

    90% as determined by SDS-PAGE.

    90% as determined by SEC-MALS.

  • 制剂(Formulation)

    Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 150 mM NaCl, pH7.5 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • 重构方法(Reconstitution)

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • 存储(Storage)

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • 质量管理控制体系(QMS)

    1. 质量管理体系(ISO, GMP)
    2. 质量优势
    3. 质控流程

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  • 电泳(SDS-PAGE)

    IgG2a Fc SDS-PAGE

    Mouse IgG2a Fc, Tag Free on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.

  • SEC-MALS

    IgG2a Fc SEC-MALS

    The purity of Mouse IgG2a Fc, Tag Free (Cat. No. IGA-M5207) is more than 90% and the molecular weight of this protein is around 48-64 kDa verified by SEC-MALS.

    Report
  • 活性(Bioactivity)-BLI

     IgG2a Fc BLI

    Loaded Mouse FCGRT&B2M Heterodimer Protein, His Tag (BLI verified) (Cat. No. FCM-M52W8) on NTA Biosensor, can bind Mouse IgG2a Fc, Tag Free(Cat. No. IGA-M5207) with an affinity constant of 5.75 nM as determined in BLI assay (ForteBio Octet Red96e) (QC tested).

    Protocol
  •  IgG2a Fc BLI

    Loaded Mouse FCGRT&B2M Heterodimer Protein, His Tag&Twin-Strep Tag (BLI verified) (Cat. No. FCM-M52W2) on NTA Biosensor, can bind Mouse IgG2a Fc, Tag Free(Cat. No. IGA-M5207) with an affinity constant of 7.73 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

    Protocol

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背景介绍

Immunoglobulin G2 (IgG2) is a member of many immunoglobulin G developed and secreted by effective B cells. In wake of cutting by pepsin, IgG is divided into two F(ab)s with one antigen binding site and a high conserved Fc segment. The Fc segment bears a highly conserved N-glycosylation site. There are two members of IgG2: IgG2a and IgG2b. It was found that IgG2a was superior to IgG1 in activating complement. The glycosylation of the circulating immunoglobulin-γ (IgG) antibody molecules changes in rheumatoid arthritis.

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