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分子别名（Synonym）
Integrin alpha 4 beta 7,ITGA4&ITGB7
表达区间及表达系统（Source）
Cynomolgus ITGA4&ITGB7 Heterodimer Protein, His Tag&Tag Free (IT7-C52W4) is expressed from human 293 cells (HEK293). It contains AA Tyr 34-Thr 977 (ITGA4) & Glu 20 - His 723 (ITGB7) (Accession # XP_005573683.2 (ITGA4) & XP_005571028.2 (ITGB7)).
Predicted N-terminus: Tyr 34 (ITGA4) & Glu 20 (ITGB7)
蛋白结构（Molecular Characterization）
Cynomolgus ITGA4&ITGB7 Heterodimer Protein, His Tag&Tag Free, produced by co-expression of ITGA4 and ITGB7, has a calculated MW of 111.6 kDa (ITGA4) & 81.6 kDa (ITGB7). Subunit ITGA4 is fused with an acidic tail at the C-terminus and followed by a polyhistidine tag and subunit ITGB7 contains no tag but a basic tail at the C-terminus. The protein migrates as 60-70 kDa, 95-120 kDa (ITGB7) and 130-150 kDa (ITGA4) when calibrated against Star Ribbon Pre-stained Protein Marker under non-reducing (NR) condition (SDS-PAGE) due to glycosylation.
偶联（Conjugate）
Unconjugated
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.
纯度（Purity）
90% as determined by SDS-PAGE.
90% as determined by SEC-MALS.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH7.5 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
质量管理控制体系（QMS）
产品展示
电泳（SDS-PAGE）
Cynomolgus ITGA4&ITGB7 Heterodimer Protein, His Tag&Tag Free on SDS-PAGE under non-reducing (NR) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%undefined.
SEC-MALS
The purity of Cynomolgus ITGA4&ITGB7 Heterodimer Protein, His Tag&Tag Free (Cat. No. IT7-C52W4) is more than 90% and the molecular weight of this protein is around 200-250 kDa verified by SEC-MALS.
Report
活性（Bioactivity）-ELISA
Immobilized Cynomolgus ITGA4&ITGB7 Heterodimer Protein, His Tag&Tag Free (Cat. No. IT7-C52W4) at 1 μg/mL (100 μL/well) can bind Anti-Human Integrin α 4 β 7 MAb, Human IgG1(ADCC Disable) with a linear range of 0.0005-0.125 μg/mL (QC tested).
Protocol
Immobilized Cynomolgus ITGA4&ITGB7 Heterodimer Protein, His Tag&Tag Free (Cat. No. IT7-C52W4) at 5 μg/mL (100 μL/well) can bind Vedolizumab with a linear range of 0.06-1 ng/mL (Routinely tested).
Protocol
Immobilized Cynomolgus ITGA4&ITGB7 Heterodimer Protein, His Tag&Tag Free (Cat. No. IT7-C52W4) at 5 μg/mL (100 μL/well) can bind Human MAdCAM-1, Fc Tag (Cat. No. MAM-H5253) with a linear range of 0.06-4 ng/mL (Routinely tested).
Protocol
活性（Bioactivity）-SPR
Human MAdCAM-1, Fc Tag (Cat. No. MAM-H5253) captured on Protein A Chip can bind Cynomolgus ITGA4&ITGB7 Heterodimer Protein, His Tag&Tag Free (Cat. No. IT7-C52W4) with an affinity constant of 5.77 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
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