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Human Laminin 411 Protein, premium grade

  • GMP version undefined is now available for seamless transition.
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  • 产品描述(Product Details)

    Laminin 411 is a key component of the basement membrane and is involved in the regulation of cell differentiation. Laminin 411 fragment consists of the C-terminal end of the alpha 4, beta 1, and gamma 1 chains.

  • 产品参数(Key parameter)

    Purity (SDS PAGE)
    > 90%
    Mycoplasma Test
    Negative
    Sterility Test
    Negative
    Endotoxin Test
    <0.1 EU per μg
  • 重构方法(Reconstitution)

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • 制剂(Formulation)

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • 重构方法(Reconstitution)

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • 存储(Storage)

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 24 months in lyophilized state;
    2. -70°C for 12 months under sterile conditions after reconstitution;
    3. 2-8°C for 3 months under sterile conditions after reconstitution.
  • 质量管理控制体系(QMS)

    1. 质量管理体系(ISO, GMP)
    2. 质量优势
    3. 质控流程

产品展示

  • 活性(Bioactivity)-ELISA

     Laminin 411 ELISA

    Immobilized Human ITGA6&ITGB1 Heterodimer Protein, His Tag&Tag Free (Cat. No. IT1-H52W7) at 5 μg/mL (100 μL/well) can bind Human Laminin 411 Protein, premium grade (Cat. No. LAT-H5263) with a linear range of 0.1-2 ng/mL (QC tested).

    Protocol
  •  Laminin 411 ELISA

    Immobilized Human ITGA7X2B&ITGB1 Heterodimer Protein, His Tag&Tag Free (Cat. No. IT1-H52W8) at 5 μg/mL (100 μL/well) can bind Human Laminin 411 Protein, premium grade (Cat. No. LAT-H5263) with a linear range of 0.01-0.625 μg/mL (Routinely tested).

    Protocol
  • 活性(Bioactivity)-SPR

     Laminin 411 SPR

    Human Laminin 411 Protein, premium grade (Cat. No. LAT-H5263) immobilized on CM5 Chip can bind Human ITGA6&ITGB1 Heterodimer Protein, His Tag&Tag Free (Cat. No. IT1-H52W7) with an affinity constant of 29.3 nM as determined in a SPR assay (Biacore 8K) (QC tested).

    Protocol
  •  Laminin 411 SPR

    Human Laminin 411 Protein, premium grade (Cat. No. LAT-H5263) immobilized on CM5 Chip can bind Human ITGA7X2B&ITGB1 Heterodimer Protein, His Tag&Tag Free (Cat. No. IT1-H52W8) with an affinity constant of 17.4 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).

    Protocol
  •  Laminin 411 SPR

    Human MCAM, His Tag (Cat. No. CD6-H5229) immobilized on CM5 Chip can bind Human Laminin 411 Protein, premium grade (Cat. No. LAT-H5263) with an affinity constant of 0.115 µM as determined in a SPR assay (Biacore 8K) (Routinely tested).

    Protocol
  •  Laminin 411 SPR

    Human MCAM, His Tag (Cat. No. CD6-H5229) captured on CM5 chip via anti-His antibody can bind Human Laminin 411 Protein, premium grade (Cat. No. LAT-H5263) with an affinity constant of 27.9 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).

    Protocol
  •  Laminin 411 SPR

    Human Laminin 411 Protein, premium grade (Cat. No. LAT-H5263) captured on Protein A Chip can bind Human MCAM, His Tag (Cat. No. CD6-H5229) with an affinity constant of 107 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).

    Protocol
  • 活性(Bioactivity)-BLI

     Laminin 411 BLI

    Loaded Human Laminin 411 Protein, premium grade (Cat. No. LAT-H5263) on Protein A Biosensor, can bind Human ITGA6&ITGB1 Heterodimer Protein, His Tag&Tag Free (Cat. No. IT1-H52W7) with an affinity constant of 26.9 nM as determined in BLI assay (ForteBio Octet Red96e) (QC tested).

    Protocol

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背景介绍

Laminin 411 is a key component of the basement membrane and is involved in the regulation of cell differentiation. Laminin 411 fragment consists of the C-terminal end of the alpha 4, beta 1, and gamma 1 chains.

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